高压氧对脆性X综合征小鼠异常行为影响的初步观察  

Effects of hyperbaric oxygen on the changes in behavior of mice with fragile X syndrome

作　　者：罗朝文 梁明坚 郭雪雅 王美仪 邓玉婷 邓小燕 陈盛强 胡楠 黄梅秀 Luo Chaowen;Liang Mingjian;Guo Xueya;Wang Meiyi;Deng Yuting;Deng Xiaoyan;Chen Shengqiang;Hu Nan;Huang Meixiu(Jinyu Medical School of Laboratory Medicine,Guangzhou Medical University,Guangzhou 511436,China)

机构地区：[1]广州医科大学金域检验学院,广州511436 [2]广州医科大学附属第二医院

出　　处：《中华航海医学与高气压医学杂志》2019年第6期545-548,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：国家自然科学基金(31771327);广州市卫生健康科技项目(20191A011077);广州市教育局创新学术团队项目(1201610032)。

摘　　要：目的探究高压氧(HBO)对脆性X综合征(FXS)模型小鼠异常行为的影响,为下一步HBO治疗FXS提供理论依据和实验支持。方法将20只Fmr1基因敲除小鼠采用数字表法随机分为HBO治疗组和对照模型组,每组10只。对HBO治疗组小鼠进行共20次HBO干预;对照模型组小鼠不作任何处理,正常饲养。21 d后对2组小鼠进行高架十字迷宫实验、旷场实验和三箱社交实验。结果HBO治疗组小鼠与对照模型组小鼠相比,在高架十字迷宫实验中,进入闭臂次数[(14.44±4.53)vs.(26.90±5.23)次]和进入闭臂次数百分比[(30.06±3.37)%vs.(57.73±7.97)%]显著减少(P<0.01),进入开臂次数[(35.00±5.25)vs.(19.45±3.61)次]和进入开臂次数百分比显著增加[(69.94±7.77)%vs.(42.27±7.97)%](P<0.01);在旷场实验中,旷场外周区活动路程[(12254.39±1860.08)vs.(7446.85±1292.22)cm]和活动总路程[(13014.37±1944.84)vs.(8051.60±1420.88)cm]显著增加(P<0.01);在三箱社交实验中,与熟悉小鼠接触次数[(37.55±19.13)次]与陌生小鼠接触次数[(18.64±10.60)次]相比显著增加[(46.45±15.65)vs.(18.57±17.83)次](P<0.01)。结论HBO能使FXS模型小鼠异常的焦虑与社交行为得到显著改善,提示HBO治疗有望成为治疗FXS的新手段。Objective To investigate the effects of hyperbaric oxygen(HBO)on the behavior of mice with fragile X syndrome FXS,so as to provide theoretical evidence and experimental support for further clinical experiment and research of possible mechanism involved for the treatment of mice with FXS.Methods Twenty Fmr1 gene knockout mice were randomly divided into the HBO group and the control group,each consisting of 10 mice.The mice in the HBO group received HBO intervention for a total of 20 sessions,while the control group was left there without any treatment,except normal feeding.After 21 days,the elevated maze test,open field test and 3-chamber social approach test were performed in the animals of the 2 groups.Results Compared with the control group,the number and percentage of entrance in the elevated maze test for the HBO group significantly decreased in the close arm[(14.44±4.53)vs.(26.90±5.23)times][(30.06±3.37)%vs.(57.73±7.97)%](P<0.01),and the number and percentage of entrance for the HBO group significantly decreased in the open arm[(35.00±5.25)vs.(19.45±3.61)times][(69.94±7.77)%vs.(42.27±7.97)%](P<0.01).In the open field test,the moving distance around the open field[(12254.39±1860.08)vs.(7446.85±1292.22)cm]and the total moving distance[(13014.37±1944.84)vs.(8051.60±1420.88)cm]all significantly increased for the HBO group(P<0.01).In the 3-chamber social test,the number of coming into contact with both familiar and unfamiliar mice all greatly increased[(46.45±15.65)vs.(18.57±17.83)times][(37.55±19.13)vs.(18.64±10.60)times](P<0.01).Conclusion HBO could obviously improve the anxiety and abnormal social behavior of the mice with FXS,suggesting that HBO therapy might be a new approach for the treatment of FXS.

关 键 词：高压氧 脆性X综合征 FMR1基因敲除小鼠 行为 

分 类 号：R[医药卫生]