人参多糖对氧化应激损伤肝细胞的保护作用机制研究  

Mechanism of Protective Effect of Ginseng Polysaccharide on Hepatocytes Induced by Oxidative Stress

作　　者：杨松 王隶书[1,2] 刘美辰[1] 陈艳艳 白雪媛[1] 王思明[1] YANG Song;WANG Li-shu;LIU Mei-chen;CHEN Yan-yan;BAI Xue-yuan;WANG Si-ming(Changchun University of Traditional Chinese Medicine,Changchun 130117,China;Jilin Provincial Academy of Chinese Medicine,Changchun 130012,China)

机构地区：[1]长春中医药大学,吉林长春130117 [2]吉林省中医药科学院,吉林长春130012

出　　处：《食品工业科技》2020年第5期280-285,292,共7页Science and Technology of Food Industry

基　　金：人参产业关键技术研究及大健康产品开发,“十三五”国家重点研发计划(2017YFC1702100);国家自然科学基金青年基金(81703663)

摘　　要：目的:以氧化应激损伤模型,通过对人参中物质基础的筛选,研究其对氧化应激造成的肝细胞损伤的保护作用及其可能的作用机制的初步探讨。方法:采用浓度为25μmol/L的过氧化氢溶液建立肝细胞损伤模型,对人参中总蛋白、总多糖、总皂苷的保护作用进行筛选。在此基础上,采用流式细胞术检测其凋亡程度、线粒体膜电位的改变,活性氧的含量变化。并采用ELISA法测肝糖原,超氧化物歧化酶(SOD)、丙二醛(MDA)、葡萄糖-6-磷酸酶(G6P)、磷酸烯醇式丙酮酸羧激酶(PEPCK)及ATP酶等指标的变化情况。结果:经过氧化氢诱导后的肝细胞,细胞活力极显著降低(P<0.01),通过对比人参中活性成分,发现人参多糖的保护作用较强,且呈现浓度依赖性;与模型组比较,人参多糖高剂量组大鼠肝细胞中MDA含量非常显著降低(P<0.001);中、高剂量组大鼠肝细胞中SOD含量显著升高(P<0.05);低剂量组大鼠肝细胞中G6P含量显著升高(P<0.05),中、高剂量组中G6P含量极显著升高(P<0.01);中剂量组大鼠肝细胞中PEPCK的含量显著升高(P<0.05),高剂量组中PEPCK的含量极显著升高(P<0.01);低、中剂量组大鼠肝细胞中ATP酶含量极显著升高(P<0.01);中、高剂量组大鼠肝细胞中肝糖原含量显著升高(P<0.05)。结论:人参多糖通过提高肝细胞中ATP酶的活力,升高线粒体膜电位来恢复肝细胞线粒体的功能,通过恢复G6P与PEPCK的含量来恢复肝脏糖异生的功能,同时通过升高SOD,降低MDA来减弱氧化应激带来的损伤,为后续研究氧化应激造成肝损伤提供一定的基础。Objective:Based on the oxidative stress injury model,the protective effect of oxidative stress on hepatocyte injury and its possible mechanism were studied by screening the material basis of ginseng. Methods:A hepatocellular injury model was established by using a hydrogen peroxide solution with the concentration of 25 μmol/L to screen protective effects of total protein,total polysaccharide,total saponin in ginseng. On this basis,cytometry was used to detect the degree of apoptosis,changes in mitochondrial membrane potential,changes in reactive oxygen species,and changes in hepatic glycogen. Changes in hepatic glycogen,superoxide dismutase(SOD),malondialdehyde(MDA),glucose-6-phosphatase(G6 P),phosphoenolpyruvate carboxykinase(PEPCK),and atpase were observed by ELISA. Result:After liver cells induced by hydrogen peroxide,cells viability were significantly reduced(P<0.01). By comparing the active ingredients in ginseng,it was found that the protective effect of ginseng polysaccharide was stronger and showed concentration dependence. Compared with the model group,the MDA content in the liver cells of rat for the high dose group of ginseng polysaccharide was significantly decreased(P<0.001). The SOD content in the liver cells of rat for the middle dose group and the high dose group were significantly increased(P<0.05). The content of G6 P in the liver cells of rat for the low dose group was significantly increased(P<0.05),and the content of G6 P in the middle dose group and the high dose group was significantly increased(P<0.01). The content of PEPCK in the liver cells of rat for the middle dose group was significantly increased(P<0.05)and the content of PEPCK in the high dose group was significantly increased(P<0.01). The atpase content in the liver cells of rat for the low dose group and the middle dose group were significantly increased(P<0.01). The content of hepatic glycogen in the liver cells of rat for middle dose group and high dose group were significantly increased(P<0.05). Conclusion:Ginseng polysacchari

关 键 词：人参多糖 氧化应激 肝细胞 糖异生 

分 类 号：TS201.4[轻工技术与工程—食品科学]