正丙醇对浑浊红球菌PD630影响的代谢组学分析  

Metabolomics analysis of 1-propanol effect on Rhodococcus opacus PD630

作　　者：楚美云 张林尚 娄文勇[1] 宗敏华[1] 唐语谦[1,2] 徐晓飞 杨继国 CHU Mei-yun;ZHANG Lin-shang;LOU Wen-yong;ZONG Min-hua;TANG Yu-qian;XU Xiao-fei;YANG Ji-guo(College of Food Science and Engineering,South China University of Technology,Guangzhou,Guangdong 510640,China;South China Institute of Collaborative Innovation,Dongguan,Guangdong 523808,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640 [2]华南协同创新研究院,广东东莞523808

出　　处：《食品与机械》2020年第1期50-55,共6页Food and Machinery

基　　金：国家重点研发计划项目(编号：2018YFC1602106,2017YFC1601005)

摘　　要：采用超高效液相色谱串联三重四级杆飞行时间质谱法(UPLC-Tnple-TOF/MS)对正丙醇存在条件下浑浊红球菌PD630体内的代谢物进行非靶标代谢组学分析。然后对其进行多元统计分析,识别差异代谢物。研究结果显示,在48 h试验组与对照组的差异代谢物共有54种,在72 h差异代谢物有61种,主要体现在短肽减少和鳞脂的不饱和度增加。同时丙酰;辅酶A(CoA)显著性增加(P<0.001),且通过气相色谱测脂肪酸组成时发现奇数碳脂肪酸的含量从32.91%提高到79.66%,提升了2.4倍。说明正丙醇对脂肪酸的合成途径影响较大。;Ultra Performance Liquid Chromatography-Tnple-Time of Flight Mass Spectrometer(UPLC-Triple-TOF/MS)was used for non-target metabolomics analysis of metabolites in Rhodococcus opacus PD630 in the presence of 1-propanol.Then a multivariate statistical analysis as well as an identification of differential metabolites was conducted for it.The results showed that there were 54 differential metabolites between the experimental group and the control group at 48 h,and 61 differential metabolites at 72 h,The decrease of short peptides and the increase in the degree of unsaturation of phospholipids were major metabolite changes.Meanwhile,a significant increase in propionyl CoA was observed(P<0.00l).The fatty acid composition was also determined by gas chromatography.The content of odd carbon fatty acids increased from 32.91% to 79.66%,with an increase of 2.4 times.This indicated that 1-propanol also had a great influence on the synthesis pathway of fatty acids.

关 键 词：正丙醇 浑浊红球菌PD630 代谢组学 代谢物 

分 类 号：Q93[生物学—微生物学] O657.63[理学—分析化学]