香榧叶精油的化学成分、抗氧化及抑菌活性的研究  

Chemical Constituents, Antioxidant and Antibacterial Activities of Essential Oils from Torreya Grandis Leaf

作　　者：曾琳[1] 林秋美 韩成云[1] 邹一平 Zeng Lin;Lin Qiumei;Han Chengyun;Zou Yiping(College of Chemistry and Biology Engineering,Yichun University,Yichun 336000;Key Laboratory of Natural Active Pharmaceutical Constituents of Jiangxi Province)

机构地区：[1]宜春学院化学与生物工程学院,宜春336000 [2]江西省天然药物活性成分研究重点实验室,宜春学院化学与生物工程学院,宜春336000

出　　处：《中国粮油学报》2020年第2期98-104,共7页Journal of the Chinese Cereals and Oils Association

摘　　要：采用水蒸气蒸馏法提取香榧叶精油,利用气-质联用技术(GC-MS)测定其化学成分及含量,探究其抗氧化及抑菌活性,确定其最低抑菌浓度。结果表明:香榧叶精油中共鉴定出40种化学成分,占总量的99.74%,主要成分为柠檬烯、α-蒎烯、β-蒎烯、香榧酯、β-谷甾醇、月桂烯、δ-杜松烯;具有较强的清除ABTS^+自由基、DPPH自由基和羟自由基的能力,且清除能力要强于维生素C;香榧叶精油对黑曲霉菌、蜡样芽胞杆菌;枯草芽孢杆菌;铜绿假单胞菌、大肠杆菌和金黄色葡萄球菌都具有较强的抗菌活性,其最低抑菌浓度分别为:0.2、0.4、0.6、0.6、0.8、0.8 mg/mL。The essential oil of Torreya Grandis Leaf was extracted by steam distillation,and its chemical composition and content were determined by gas chromatography-mass spectrometry(GC-MS).The results showed that there were 40 kinds of chemical constituents in the essential oil of Torreya Grandis Leaf,accounting for 99.74%of the total amount.The main components included limonene,α-pinene,β-pinene,torreyagrandate,β-sitosterol,myrcene andδ-cadinene,with stronger ability of scavenging ABTS^+free radical,DPPH free radical and hydroxyl free radical than vitamin C;It had strong antibacterial activity against Aspergillus niger,Bacillus cereus,Bacillus subtilis,Pseudomonas aeruginosa,Staphylococcus aureus and Escherichia coli,and the minimum inhibitory concentrations of which were 0.2,0.4,0.6,0.6,0.8 and 0.8 mg/mL,respectively.

关 键 词：香榧叶 精油 化学成分 抗氧化性 抑菌活性 

分 类 号：R962[医药卫生—药理学]