高通量测序技术初步解析浓香型白酒窖泥细菌群落结构  

Preliminary analysis of bacterial community structure in strong-flavor Baijiu pit mud by high-throughput sequencing technology

作　　者：唐贤华[1] TANG Xianhua(Department of Alcohol and Food Engineering,Sichuan Technology&Business College,Dujiangyan 611830,China)

机构地区：[1]四川工商职业技术学院酒类与食品工程系

出　　处：《中国酿造》2020年第1期160-164,共5页China Brewing

基　　金：四川省教育厅项目(17ZB0268)

摘　　要：通过高通量测序技术初步解析了不同窖池窖泥的细菌群落结构。结果表明,窖泥细菌分为9个门类,其中厚壁菌门(Firmicutes)占据主导地位;放线菌门(Actinobacteria)在不同窖泥中的占比差异比较大。而拟杆菌门(Bacteroidetes)、绿弯菌门(Chlorflexi)、互养菌门(Synergistetes)、变形菌门(Proteobacteria)、无壁菌门(Tenericutes)、螺旋体门(Spirochaetes)、球孢菌门(Lebtisphaeria)在不同窖池窖泥中所占比例也有所差别。对窖泥细菌属分类发现,9个窖泥样品中一共分成39个属。通过主成分分析发现其中13个细菌属对窖泥来源划分的贡献度较高。相同车间窖泥中八叠球菌属(Sporobacter)、红蝽杆菌属(Coriobacterium)、梭菌属(Clostridium)、纤维素单胞菌属(Cellulomonas)、Sediminibacter、氨基杆菌属(Aminobacter)、棒杆菌属(Corynebacterium)、Sporosarcina所占比例基本相同。而脱卤素杆菌属(Dehalobacter)、醋杆菌属(Acetobacter)、互营菌属(Syntrophus)、冷单胞菌属(Psychromonas)只在7、8、9号窖泥中发现有一定比例的分布,而在其他窖泥中均未检测出。The bacterial community structure of pit mud in different pits was preliminarily analyzed by high-throughput sequencing technology.The results showed that the bacteria in the pit mud were divided into 9 phyla.Among them,the Firmicutes was dominated,and the proportion of Actinobacteria had a large of difference in different pit mud.While the proportions of Bacteroidetes,Chlorflexi,Synergistetes,Proteobacteria,Tenericutes,Spirochaetes and Lebtisphaeria in the pit mud of different pits were also different.The classification results of bacteria in the pit mud showed there were 39 genera in 9 pit mud samples.According to principal component analysis,13 bacterial genera contributed more to the classification of pit mud sources.The proportion of Sporobacter,Coriobacterium,Clostridium,Cellulomonas,Sedimenlibacter,Aminobacterium,Corynebacterium and Sporosarcina in the pit mud of same workshop was basically the same.While the Dehalobacter,Acetobacter,Syntrophus and Psychromonas were only found in pit mud NO.7,NO.8 and NO.9,and were not detected in other pit mud.

关 键 词：高通量测序 窖泥细菌 群落结构 主成分分析 

分 类 号：TS261.1[轻工技术与工程—发酵工程]