Effect of methanol extract of Schisandrae Fructus on high fat diet induced hyperlipidemic mice  

作　　者：Se-Eun Lee Hyungwoo Kim Chang-Hyun Kim Chiyeon Lim Suin Cho 

机构地区：[1]Department of Korean Medicine,School of Korean Medicine,Yangsan Campus of Pusan National University,Yangsan 50612,Republic of Korea [2]Department of Medicine,College of Medicine,Dongguk University,Goyang 10326,Republic of Korea

出　　处：《Journal of Traditional Chinese Medicine》2019年第6期818-825,共8页中医杂志（英文版）

基　　金：Supported by the 2019 Post-Doc.Development Program of Pusan National University

摘　　要：OBJECTIVE:To investigate the effects and molecular targets of Schisandrae Fructus(SF)methanol extract(SFme)in mice with hyperlipidemia induced by high fat diet.METHODS:We observed changes in body weight,blood serum content of total cholesterol,high-density lipoprotein(HDL)-cholesterol,and triglyceride.The extent of accumulation of lipid peroxide due to lipid metabolism disorder also evaluated by measuring malondialdehyde(MDA)level.In addition,after getting gene expression in hepatic tissues,target protein of SFme was identified using a protein interaction database.RESULTS:SFme significantly decreased total cholesterol and triglyceride levels without alteration of body weight in mice,and the livercontent of MDA was statistically decreased by SFme.And expression changes of cyclindependent kinase 1(Cdk1)and leucine-rich repeat kinase 2(Lrrk2)were restored by SFme.CONCLUSION:The effect of SFme on the highfat-diet induced hyperlipidemia via decreasing total cholesterol and triglyceride levels may involve the expression of Cdk1 and Lrrk2 proteins.OBJECTIVE: To investigate the effects and molecular targets of Schisandrae Fructus(SF)methanol extract(SFme) in mice with hyperlipidemia induced by high fat diet.METHODS: We observed changes in body weight,blood serum content of total cholesterol,high-density lipoprotein(HDL)-cholesterol, and triglyceride. The extent of accumulation of lipid peroxide due to lipid metabolism disorder also evaluated by measuring malondialdehyde(MDA)level. In addition, after getting gene expression in hepatic tissues, target protein of SFme was identified using a protein interaction database.RESULTS: SFme significantly decreased total cholesterol and triglyceride levels without alteration of body weight in mice, and the livercontent of MDA was statistically decreased by SFme. And expression changes of cyclindependent kinase 1(Cdk1) and leucine-rich repeat kinase 2(Lrrk2) were restored by SFme.CONCLUSION: The effect of SFme on the highfat-diet induced hyperlipidemia via decreasing total cholesterol and triglyceride levels may involve the expression of Cdk1 and Lrrk2 proteins.

关 键 词：SCHISANDRA HYPERLIPIDEMIAS OBESITY Cardiovascular diseases CDC2 protein kinase 

分 类 号：R285.5[医药卫生—中药学]