基于深度卷积神经网络对中期染色体分类的应用研究  

Application research on classification of metaphase chromosomes based on deep convolutional neural networks

作　　者：张成成 宋婕萍[1] 徐淑琴[1] 李卉 徐闰红 王小艳 张立 游齐靖 张凯[4] 林浩添[5] ZHANG Cheng-cheng;SONG Jie-ping;XU Shu-qin(Department of Clinical Laboratory,Maternal and Child Health Hospital of Hubei Province,Wuhan 430070,China)

机构地区：[1]湖北省妇幼保健院检验科,武汉430070 [2]华中科技大学同济医学院附属武汉中心医院眼科,武汉430014 [3]南京航空航天大学电子信息工程学院,江苏211106 [4]西安电子科技大学计算机科学与技术学院,陕西710071 [5]中山大学中山眼科中心,广州510060

出　　处：《中国临床新医学》2020年第2期123-126,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：武汉市卫生健康委员会医学科研项目(编号:WX19Q29)

摘　　要：目的通过深度卷积神经网络对分裂中期的染色体图像进行学习,测试其在染色体分类的准确率。方法该研究一共纳入1275例不同个体分裂中期的染色体图片,其中735例作为训练集用于深度卷积神经网络,245例作为测试集用作内部验证,245例外院数据用作外部验证;另取50例染色体图片资料,分别记录人和计算机完成染色体分类的时间及准确率。结果24分类的结果提示,分类网络在内部验证的准确率为91.22%,外部验证的准确率为91.48%;ResNet的分类效率是人工操作的1000倍以上,其准确率明显优于非遗传专科医师。结论深度卷积神经网络在染色体分类的应用价值极具有发展潜力,将有助于构建染色体核型分析自动化平台。Objective Deep convolutional neural networks was used to learn the chromosome images in the metaphase of mitosis and test its accuracy in chromosome classification. Methods A total of 1 275 chromosome images of different individual metaphase divisions were included in this study,of which 735 cases were used as training sets for deep convolutional neural network,and 245 cases were used as test sets for internal validation,and 245 cases of another hospital’s data were used as external validation. In addition,50 chromosome images were taken to record the time and accuracy of completing chromosome classification by human and computer. Results The results of 24 categories indicated that the accuracy rate of the internal validation was 91. 22% and that the accuracy rate of the external validation was 91. 48%. The efficiency of classification by Res Net was more than 1 000 times higher than that by manual operation,and its accuracy was significantly better than that of non-genetic specialists. Conclusion Deep convolutional neural networks has great potential in the application of chromosome classification and will be helpful to construct a platform for automatic chromosome karyotype analysis.

关 键 词：染色体分类 卷积神经网络 染色体核型分析 

分 类 号：TP183[自动化与计算机技术—控制理论与控制工程]