一株溶植酸磷类芽孢杆菌的分离筛选及对水稻幼苗的促生作用  

Isolation and screening of a phytate phosphate-solubilizing Paenibacillus sp. and its growthpromoting effect on rice seeding

作　　者：渠露露 彭长连[1] 李淑彬[1] QU Lu-lu;FENG Chang-lian;LI Shu-bin(College of Life Sciences,South China Normal University,Guangzhou 510631,China)

机构地区：[1]华南师范大学生命科学学院

出　　处：《应用生态学报》2020年第1期326-332,共7页Chinese Journal of Applied Ecology

基　　金：国家重点研发项目(2017YFC1200105);国家自然科学基金项目(31870374)资助~~

摘　　要：采用溶磷平板筛选和重金属耐性复筛相结合,从薇甘菊根际分离到一株兼具多重金属抗性的溶磷细菌(编号为ZLT11),16S r RNA基因序列分析显示,该菌株属于类芽孢杆菌。菌株对植酸钙和植酸的溶磷量分别为84.10和73.84 mg·L^-1,其在30℃、初始pH为9.0时对植酸钙的溶磷量最高,达95.66 mg·L^-1。菌株ZLT11能耐受≤400 mg·L^-1Pb^2+、≤100mg·L^-1Cd^2+及≤40 mg·L^-1Hg^2+,当添加植酸钙为磷源时,接种菌株ZLT11使水稻幼苗平均根长、根数、苗高和总生物量较对照幼苗分别增加106.7%、76.6%、49.0%和46.3%。菌株ZLT11还能显著促进水稻幼苗在Cd胁迫下的生长。The phosphate-solubilizing medium plate screening and heavy metal resistance rescreening were used to isolate a phosphate-solubilizing bacterium(coded ZLT11)from the rhizosphere of Mikania micrantha.Results from 16 S r RNA gene sequence analysis revealed that the strain ZLT11 belonged to Paenibacillus sp.The amount of phosphorus solubilized from calcium phytate and phytic acid by the ZLT11 was 84.10 and 73.84 mg·L^-1,respectively.The maximum phosphate solubilizing activity to calcium phytate(95.66 mg·L^-1)was at 30℃and initial pH 9.0.The strain ZLT11 displayed the tolerance to≤400 mg·L^-1 Pb^2+,≤100 mg·L^-1 Cd^2+,and≤40 mg·L^-1 Hg^2+.With calcium phytate as phosphorus source,the inoculation strain ZLT11 treatment increased the average root length,root number,seedling height and total biomass of rice seedlings by 106.7%,76.6%,49.0% and 46.3%,respectively.The strain ZLT11 could improve rice seedlings growth under Cd stress.

关 键 词：植酸磷 类芽孢杆菌 重金属耐受 水稻 

分 类 号：S511[农业科学—作物学] X172[环境科学与工程—环境科学]