柚皮苷改善CCL2所致大鼠学习记忆障碍及其机制  

Naringin improves learning and memory impairment induced by CCL2 in rats and its mechanism

作　　者：龙江宜 陈健民[1] 廖苑君 周怡俊 梁冰玉[2] 周燕[1] LONG Jiang-yi;CHEN Jian-min;LIAO Yuan-jun;ZHOU Yi-jun;LIANG Bing-yu;ZHOU Yan(Dept of Pharmacology,Guangxi Medical University,Nanning 530021,China;Guangxi Key Lab of AIDS Prevention and Treatment,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西医科大学广西艾滋病防治研究重点实验室,广西南宁530021

出　　处：《中国药理学通报》2020年第3期372-379,共8页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81660213,81360192);广西自然科学基金资助项目(No 2017GXNSFAA198187,2018GXNSFAA281325);广西一流学科(药学)建设项目(No GXFCDP-PS-2018)。

摘　　要：目的探究柚皮苷改善CCL2诱导大鼠学习记忆障碍及其机制。方法56只SD大鼠随机分为空白组、假手术组、模型组(CCL2)、阳性药(CCL2+memantine)组、柚皮苷低(CCL2+25 mg·kg^-1柚皮苷)、中(CCL2+50 mg·kg^-1柚皮苷)、高(CCL2+100 mg·kg^-1柚皮苷)剂量组。除空白、假手术组外,各组均通过脑部定位将CCL2注射至大鼠海马制作学习记忆障碍模型。Morris水迷宫检测各组大鼠的学习和记忆水平;HE染色观察大鼠海马CA1区神经元形态;试剂盒检测海马SOD、GSH-PX活力和MDA含量;qRT-PCR检测凋亡基因caspase-3、caspase-8、Bax、Bcl-2 mRNA的相对表达。结果与模型组相比,各柚皮苷给药组大鼠逃避潜伏期及游泳路程均明显减少,平台穿越次数增加;海马CA1区神经元排列紧密且形态良好;SOD、GSH-PX活力升高,MDA含量降低;凋亡基因caspase-8、caspase-3、Bax mRNA相对表达量减少;Bcl-2表达量增加。结论柚皮苷能明显改善CCL2所致大鼠学习记忆功能障碍,其机制与柚皮苷的抗氧化和抗凋亡效应有关。Aim To explore the protective effect of na-ringin on CCL2-induced learning and memory impair-ment and its mechanisms.Methods Fifty-six male SD rats were equally divided into control,sham,model(5 ng CCL2),positive drug(5 ng CCL2+meman-tine),naringin low(5 ng CCL2+25 mg·kg^-1 narin-gin),middle(5 ng CCL2+50 mg·kg^-1 naringin)and high(5 ng CCL2+100 mg·kg^-1 naringin)groups,with eight SD rats in a group.Each group re-ceived stereotaxic surgery to inject CCL2 into bilateral hippocampus.Morris water maze was used to assess learning and memory.Commercial kits were used to measure the activity of SOD,GSH-PX and the content of MDA in hippocampus.qRT-PCR was performed to detect the relative mRNA expression of apoptois-associ-ated genes caspase-3,caspase-8,Bax and Bcl-2.Re-sults Compared with model group,the escape latency and swimming distance in drug treatment groups were significantly reduced;the number of platform crossing times significantly increased;the neurons of hippocam-pal CA1 were better than those of model group;the ac-tivities of SOD and GSH-PX were boosted while the content of MDA decreased;the relative expression lev-els of caspase-3,caspase-8,Bax decreased while the Bcl-2 increased.Conclusions Naringin can im prove learning and memory impairment induced by CCL2,and the underlying mechanisms may involve its role of anti-oxidation and anti-apoptosis.

关 键 词：柚皮苷 CCL2 海马 学习记忆 氧化应激 凋亡 

分 类 号：R-332[医药卫生] R284.1