氮源对阿维菌素合成的影响及基于二氧化碳释放速率的阿维菌素发酵调控  

Effect of nitrogen on avermectins biosynthesis and its fermentation optimization based on carbon-dioxide evolution rate

作　　者：徐瑶 吴涛 郭美锦[1] 庄英萍[1] 王得明 樊乐 Yao Xu;Tao Wu;Meijin Guo;Yingping Zhuang;Deming Wang;Le Fan(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;Qilu Pharmaceutical(Inner Mongolia)Co.,Ltd.,Hohhot 010000,Inner Mongolia,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]齐鲁制药(内蒙古)有限公司,内蒙古呼和浩特010000

出　　处：《生物工程学报》2020年第2期287-294,共8页Chinese Journal of Biotechnology

摘　　要：中国是世界上最大的也是唯一的阿维菌素原料生产国,但在工业规模生产中与同类型大环内酯类抗生素相比其产量相对偏低。文中通过研究不同氮源对阿维链霉菌生长、代谢的影响,发现氮源在发酵中后期对菌丝活性、菌丝浓度以及阿维菌素B1a的合成都有较为显著的影响。在100 L生物反应器中,于发酵中后期基于二氧化碳释放速率(CER)控制补入酵母粉,效价达到8697mg/L,与原工艺相比,提高了26.9%。这一结论若在实际工业生产中应用,有望带来实际的经济效益。China is now the largest and only producer of avermectin in the world. However, its current yield is still lower than other similar antibiotics. Therefore, we studied the effect of nitrogen on the growth and the synthesis ability of B1 a to improve the overall yield. Nitrogen had significant effects on the cell activity, PMV of Streptomyces avermitilis and the synthesis of B1 a in the middle and later phase of fermentation. Additional feeding yeast powder based on carbon-dioxide evolution rate in a 100-L bioreactor significantly improved the synthesis of B1 a. The production of avermectin reached 8 697 mg/L, 26.9% higher than the original process. In short, this study will serve in production enhancement of avermectin at industrial production.

关 键 词：阿维链霉菌 阿维菌素 酵母粉 二氧化碳释放速率 

分 类 号：TQ927[轻工技术与工程—发酵工程]