金针菇贮藏保鲜期间表面细菌菌相变化  

Bacterial Microflora on Exterior of Flammulina velutipes Fruiting Bodies during Chilled Storage

作　　者：潘欣圆 杨娟 施乐乐 蔡志英 李若熙 陈炳智[1,4] 江玉姬[1,4] PAN Xinyuan;YANG Juan;SHI Lele;CAI Zhiying;LI Ruoxi;CHEN Bingzhi;JIANG Yuji(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Edible Fungi Technical Popularized Station,Fuzhou,Fujian 350000,China;Longhai Agricultural Bureau,Longhai,Fujian 363100,China;Mycological Research Center of Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福建省食用菌技术推广总站,福建福州350002 [3]龙海市农业局,福建龙海363100 [4]福建农林大学菌物研究中心,福建福州350002

出　　处：《食用菌学报》2020年第1期29-35,共7页Acta Edulis Fungi

基　　金：福建农林大学校创新基金(KF2015050)。

摘　　要：采用传统平板培养法与宏基因组测序法,对4℃贮藏保鲜条件下金针菇(Flammulina velutipes)表面细菌数量及种类进行分析。传统平板培养法共分离到3个门6个属的细菌,其中美洲爱文氏菌属和乳酸乳球菌属的细菌在不同贮藏时间均被分离到。宏基因组测序法共分析鉴定出13个门80个属的细菌,主要为厚壁菌门的乳酸乳球菌属和魏斯氏菌属,变形菌门的耶尔森氏菌属。研究表明:4℃贮藏条件下,金针菇表面细菌群落结构具有丰富的多样性,随着贮藏时间延长,菌落总数不断增加,而细菌种类数目下降,乳酸乳球菌是金针菇4℃贮藏保鲜期间的优势菌。Using the traditional culture plate separation and the metagenomics sequencing method,exterior bacterial microflora on Flammulina velutipes fruiting bodies stored at 4℃in vacuum sealed polypropylene bags was studied.The results showed that a total of 6 genera belonging to 3 phyla were isolated from different storage time sampling by the traditional culture plate separation method;and the dominant taxa,Lactococcus and Ewingella,were identified throughout the duration of the entire tested storage time.By metagenomics sequencing,a total of 80 genera belonging to 13 phyla were identified,and the dominant taxa were Lactococcus and Weissella of Firmicutes,and Yersinia of Proteobacteria.During the storage at 4℃,a rich bacteria diversity was initially detected,and then the diversity decreased over time in contrast to an increasing overall bacterial population.During the entire preservation period,the dominant and the major spoilage bacterium was Lactococcus lactis.

关 键 词：金针菇 平板培养 宏基因组测序 细菌种群结构 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]