基于转录组技术研究藻蓝蛋白在抑制非小细胞肺癌中的调控作用  

RNA-seq Transcriptomic Analysis of Phycocyanin in Regulating the Inhibition of Non-small Cell Lung Cancer Cells

作　　者：郝帅[1] 李爽[1] 王静[1] 吴婷婷 张佳雯 王成涛[1] HAO Shuai;LI Shuang;WANG Jing;WU Tingting;ZHANG Jiawen;WANG Chengtao(Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Engineering and Technology Research Center of Food Additives,School of Food and Health,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]北京市食品营养与人类健康高精尖创新中心,北京市食品添加剂工程技术研究中心,北京工商大学食品与健康学院,北京100048

出　　处：《食品科学》2020年第5期93-101,共9页Food Science

基　　金：国家自然科学基金青年科学基金项目(31701575);国家自然科学基金面上项目(31571801);“十三五”国家重点研发计划重点专项(2016YFD0400502-02);科技创新服务能力建设-科技成果转化-提升计划项目(PXM2016-014213-000034)。

摘　　要：藻蓝蛋白是我国认可的天然食品着色剂,广泛用于多种食品产业中,同时也是一种天然功能性食用因子,具有优良的抗肿瘤能力。本研究通过高通量转录组学技术对藻蓝蛋白处理前后的H460细胞进行了深入分析,结果表明,2532个基因的转录水平受到了藻蓝蛋白的影响,其中藻蓝蛋白处理后表达上调的基因有1491个,表达下调的基因有1041个;GO分析结果显示,这些差异基因主要参与了细胞增殖调控过程、细胞凋亡过程的调控、细胞因子应答反应等生物学过程;聚类分析结果显示,Cdk2、Cdc25、PCNA、TLR4等影响细胞增殖的基因均出现了显著下调(P<0.05);p27、p21等抑制细胞增殖的基因出现了显著上调(P<0.05);Bcl-2、NKD1等癌基因出现了极显著下调(P<0.01);而CCT6、GBP2等促凋亡基因出现了极显著上调(P<0.01),并且这些基因的变化情况与定量聚合酶链式反应结果一致;KEGG信号通路分析显示PI3K-Akt信号通路是藻蓝蛋白参与调控H460细胞的重要途径。本研究基于转录组学技术,深入分析了藻蓝蛋白对非小细胞肺癌H460细胞的调控机制,为非小细胞肺癌的靶向治疗和抗肿瘤类功能性食品因子的开发利用提供了理论参考。Phycocyanin is a natural food colorant used in many food industries in China. It is also a natural functional food protein with good antitumor capacity. In the present study, the high-throughput RNA-seq analysis showed that phycocyanin affected the expression levels of 2 532 genes, including 1 491 up-regulated ones and 1 041 down-regulated ones. GO analysis showed that these differentially expressed genes mainly participated in cell proliferation, apoptosis and cytokine responses. Cluster analysis showed that proliferation-related genes such as Cdk2, Cdc25, PCNA and TLR4 were down-regulated, whilst p27 and p21 were up-regulated after phycocyanin treatment. Meanwhile, apoptosis-related genes such as Bcl-2 and NKD1 were significantly down-regulated, whereas CCT6 and GBP2 were significantly up-regulated. KEGG pathway analysis showed that PI3 K-Akt signaling was one of the important pathways through which phycocyanin participated in regulating H460 cells. By revealing the potential mechanism of action of phycocyanin in non-small cell lung cancer H460 cells, the present study may provide a theoretical foundation for the development of targeted therapeutics against non-small cell lung cancer and of functional food factors against tumor cells.

关 键 词：藻蓝蛋白 非小细胞肺癌 H460细胞 转录组学 细胞增殖 细胞凋亡 

分 类 号：TS202.3[轻工技术与工程—食品科学] R151.1[轻工技术与工程—食品科学与工程]