实时荧光定量PCR鉴定肉制品中牛源性成分及其含量  

Identification of Bovine Derived Components and Contents in Products by Real-time Fluorescent Quantitative PCR

作　　者：史莹莹 康雨薇 邵俊锋 郭娟 许慧卿[1] SHI Ying-ying;KANG Yu-wei;SHAO Jun-feng;GUO Juan;XU Hui-qing(College of Food Science and Technology,Yangzhou University,Yangzhou 225127,Jiangsu,China)

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225127

出　　处：《食品研究与开发》2020年第6期158-163,共6页Food Research and Development

摘　　要：建立一种快速、准确鉴定牛肉制品中牛源性成分并且量化牛肉成分含量的方法。以牛线粒体细胞色素b基因为靶基因,设计出具有特异性引物。选择真核生物核糖体16SrDNA为内参基因,采用实时荧光相对定量法。牛肉质量百分比的对数值与之对应的循环阈值差值△Ct呈良好线性关系。标准曲线回归公式为y=-3.3645x+0.8737,R2=0.9926,扩增效率达98.25%。通过模拟混合样品对标准曲线进行质量评估,证明该方法适用于对牛肉成分的鉴定以及含量的检测,为量化肉制品中牛肉成分研究提供参考意见。A rapid and accurate method for identifying bovine-derived components in beef products and quantifying the content of beef components was established.The bovine mitochondrial cytochrome b gene was used as a target gene to design specific primers.The eukaryotic ribosomal 16 SrDNA was selected as an internal reference gene,and real-time fluorescence relative quantification was used.The logarithm of the percentage of beef mass had a good linear relationship with the corresponding cycle threshold differenceΔCt.The standard curve regression formula was y=-3.3645 x+0.8737,R2=0.9926,and the amplification efficiency reached98.25%.The quality of the standard curve was evaluated by simulating mixed samples,which proved that the method was suitable for the identification and content detection of beef components.It could provide reference comments for research on quantifying beef composition in meat products.

关 键 词：实时荧光定量多聚核苷酸链式反应 牛肉 线粒体细胞色素B 相对定量 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]