Decreasing intraocular pressure significantly improves retinal vessel density,cytoarchitecture and visual function in rodent oxygen induced retinopathy  

作　　者：Rui Du Xu Wang Ke Shen Shigang He 

机构地区：[1]School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China [2]Institute of Natural Sciences,Shanghai Jiao Tong University,Shanghai 200240,China [3]Bio-X Institute,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Science China(Life Sciences)》2020年第2期290-300,共11页中国科学（生命科学英文版）

基　　金：funded by a key project of the National Natural Science Foundation of China (31030036) to SH。

摘　　要：We attempted to explore a noninvasive,easily applicable and economically affordable therapy for retinopathy of prematurity(ROP).Rat pups were raised in 80%oxygen from postnatal day 7 to P12,and returned to room air.Travoprost eye drops were administered twice a day for 7 days,to reduce intraocular pressure(IOP)by about 20%.Immunohistochemical staining was performed to visualize vessel endothelial cells,to analyze retinal neurons and cytoarchitecture.Behavioral experiments were carried out to test visual acuity and contrast sensitivity.At the end of the 7-day treatment,the number of vessels extending to the vitreous body was significantly reduced and retinal vessel density increased.This improvement was maintained to the end of the12 th week.In the central retina of the model group,the horizontal cells were completely wiped out,the outer plexiform layer was undetectable,and the rod bipolar cell dendrites sprouted into the outer nuclear layer.The treatment partially reverted these architectural changes.Most importantly,behavioral experiments revealed significantly improved visual acuity and contrast sensitivity in the treated group.Therefore,reducing IOP could potentially serve as a safe and economical measure to treat ROP.We attempted to explore a noninvasive, easily applicable and economically affordable therapy for retinopathy of prematurity(ROP). Rat pups were raised in 80% oxygen from postnatal day 7 to P12, and returned to room air. Travoprost eye drops were administered twice a day for 7 days, to reduce intraocular pressure(IOP) by about 20%. Immunohistochemical staining was performed to visualize vessel endothelial cells, to analyze retinal neurons and cytoarchitecture. Behavioral experiments were carried out to test visual acuity and contrast sensitivity. At the end of the 7-day treatment, the number of vessels extending to the vitreous body was significantly reduced and retinal vessel density increased. This improvement was maintained to the end of the12 th week. In the central retina of the model group, the horizontal cells were completely wiped out, the outer plexiform layer was undetectable, and the rod bipolar cell dendrites sprouted into the outer nuclear layer. The treatment partially reverted these architectural changes. Most importantly, behavioral experiments revealed significantly improved visual acuity and contrast sensitivity in the treated group. Therefore, reducing IOP could potentially serve as a safe and economical measure to treat ROP.

关 键 词：OXYGEN INDUCED RETINOPATHY vessel plasticity visual ACUITY contrast sensitivity rat 

分 类 号：R774.1[医药卫生—眼科]