结直肠癌中C-erbB-2基因扩增与KRAS基因突变的意义  

Significance of C-erbB-2 gene amplification and KRAS gene mutation in colorectal cancer

作　　者：潘理会[1] 李育庄 李春辉[2] Pan Lihui;Li Yuzhuang;Li Chunhui(Department of Biomedical Engineering,Chengde Medical College,Chengde 067000,China;Pathology Department,Affiliated Hospital of Chengde Medical College,Chengde 067000,China)

机构地区：[1]承德医学院生物医学工程系,河北承德067000 [2]承德医学院附属医院病理科,河北承德067000

出　　处：《实用肿瘤杂志》2020年第1期48-52,共5页Journal of Practical Oncology

基　　金：河北省医学适用技术跟踪资助项目(G2018070)。

摘　　要：目的探讨结直肠癌(colorectal cancer,CRC)中人类表皮生长因子受体2(human epidermal growth factor receptor 2,HER-2,又为C-erbB-2)基因与鼠类肉瘤病毒癌基因(Kirsten rat sarcoma viral oncogene,KRAS)突变情况及其与临床病理特征的关系。方法回顾性分析238例结直肠癌患者相关临床资料。采用荧光原位杂交(fluorescence in situ hybridization,FISH)法检测结直肠癌组织中的C-erbB-2扩增情况。采用荧光实时定量聚合酶链式反应(real time quantity polymerase chain reaction,qRT-PCR)法检测KRAS的突变情况,并分析其与临床病理特征之间的关系。结果结直肠癌中C-erbB-2总扩增率为1.7%(4/238),KRAS总突变率为36.9%(88/238)。C-erbB-2扩增在年龄、性别、肿瘤发生部位、组织学分型、分化程度、淋巴结转移、Duke's分期及浸润深度等方面比较,差异均无统计学意义(均P>0.05)。KRAS突变在淋巴结转移及Duke's分期方面比较,差异均具有统计学意义(均P<0.05)。C-erbB-2与KRAS二者表达无相关关系(r=0.086,P=0.184)。结论结直肠癌发生和发展过程中C-erbB-2扩增与KRAS突变无相关性,KRAS突变与淋巴结转移和Duke's分期有关。Objective To investigate the mutation of human epidermal growth factor receptor 2(C-erbB-2)gene and Kirsten rat sarcoma viral oncogene(KRAS)gene in colorectal cancer and its relationship with clinicopathological features.Methods The clinical data of 238 patients with colorectal cancer were retrospectively analyzed.Fluorescence in situ hybridization(FISH)was used to detect C-erbB-2 amplification in colorectal cancer tissues.The real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the mutation of KRAS,and the relationship between clinical and pathological features was analyzed.Results The total amplification rate of C-erbB-2 in colorectal cancer was 1.7%(4/238),and the total KRAS mutation rate was 36.9%(88/238).C-erbB-2 amplification was not statistically significant in terms of age,sex,tumor location,histological classification,degree of differentiation,lymph node metastasis,Duke's stage and depth of invasion(all P>0.05).KRAS mutations were statistically significant in lymph node metastasis and Duke's stage(both P<0.05).There was no correlation between the expression of C-erbB-2 and KRAS(r=0.086,P=0.184).Conclusions There is no significant correlation between C-erbB-2 amplification and KRAS mutation during the occurrence and development of colorectal cancer.KRAS mutation is related to lymph node metastasis and Duke′s stage.

关 键 词：结直肠肿瘤/病理学 基因 ERBB-2 受体 表皮生长因子 基因 ras 基因扩增 突变 原位杂交 荧光 

分 类 号：R735.3[医药卫生—肿瘤] K730.2[医药卫生—临床医学]