近日节律紊乱影响大鼠更年期综合征的心理生理学观察  

Psychophysiological observation on the effects of circadian rhythm disorder on climacteric syndrome model rats

作　　者：黄蓉 吴庆庆 李光建 祝金津 汪萌芽[1] HUANG Rong;WU Qingqing;LI Guangjian;ZHU Jinjin;WANG Mengya(Cell Electrophysiology Laboratory,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院细胞电生理研究室,安徽芜湖241002 [2]皖南医学院启明星小组,安徽芜湖241002

出　　处：《皖南医学院学报》2020年第1期1-5,共5页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(31271155);皖南医学院大学生科研资助金项目(WK2016S25)。

摘　　要：目的:观察近日节律紊乱对更年期综合征模型大鼠的影响,以探究更年期综合征的可能心理生理学机制。方法:取雌性SD大鼠35只,随机分为正常组(n=7)、假手术组(n=7)、模型组(n=7)、近日节律紊乱组(n=7)、模型+近日节律紊乱组(n=7)。采用双侧卵巢摘除术建立更年期综合征大鼠模型,以24 h光照为近日节律紊乱干预。通过多通道心理生理记录技术,对更年期综合征相关核团室旁核(PaPo)和近日节律相关核团视交叉上核(SCh)的生物电活动进行同步细胞外记录和相关生理指标联合记录和分析。结果:①模型组、近日节律紊乱组、模型+近日节律紊乱组大鼠的PaPo放电频率高于假手术组和正常组(P<0.05或P<0.01);②正常组大鼠的SCh放电频率低于其余4组(P<0.01);③模型组大鼠PaPo的beta和fast振荡能量高于其余4组(P<0.05);④假手术组大鼠SCh的delta和theta振荡能量高于其余4组(P<0.05)。⑤仅模型+近日节律紊乱组大鼠PaPo和SCh的放电频率呈高度正相关(r=0.902,P=0.014)。结论:更年期综合征模型大鼠可能涉及PaPo和SCh活动变化机制,近日节律紊乱可能通过改变PaPo和SCh的相关性而影响更年期综合征的心理生理学特征。Objective:To investigate the effects of circadian rhythm disorder on climacteric syndrome in rat models as well as the potential psychophysiological mechanisms.Methods:Thirty-five female SD rats were evenly randomized into group of normal,sham operation,model,dysrhythmia and model+dysrhythmia.Rat models of climacteric syndrome were prepared by surgical bilateral ovarian ablation,and dysrhythmia was developed by 24-hour light.Simultaneous extracellular recordings of bioelectrical activities in the paraventricular nucleus(PaPo)and the suprachiasmatic nucleus(SCh)were maintained,and the related physiological functions were monitored.Results:①The PaPo discharge frequency in model rats,dysrhythmia and model+dysrhythmia groups was higher than that of sham operation and normal groups(P<0.05 or P<0.01);②The SCh discharge frequency was lower in normal group rats than in the other 4 groups(P<0.01);③The power of beta and fast rhythm of PaPo in model rat group was higher than that of the other 4 groups(P<0.05);④The power of delta and theta rhythm of SCh was lower in sham operation group than in the other 4 groups(P<0.05);⑤The discharge frequency of PaPo and SCh in the model+dysrhythmia group was presented with highly positive correlation(r=0.902,P=0.014).Conclusion:Climacteric syndrome in model rats may be involved in mechanisms of PaPo and SCh activity changes,and dysrhythmia may affect the psychophysiological picture of climacteric syndrome by changing the correlation between PaPo and SCh acitivity.

关 键 词：更年期综合征 近日节律紊乱 室旁核 视交叉上核 心理生理学 

分 类 号：R-332[医药卫生] R338.2