A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice  

作　　者：Jiangman He Chi Zhang Huiling Dai Huan Liu Xiaowei Zhang Jun Yang Xi Chen Yayun Zhu Dapeng Wang Xiaofeng Qi Weichao Li Zhihui Wang Guoyong An Nan YU Zuhua He Yong-Fei Wang Youli Xiao Peng Zhang Ertao Wang 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China [2]University of Chinese Academy of Sciences,Beijing 100039,China [3]College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China [4]Collaborative Innovation Center of Crop Stress Biology,Henan Province,Institute of Plant Stress Biology,State Key Laboratory of Cotton Biology,Department of Biology,Henan University,Kaifeng 475001,China

出　　处：《Molecular Plant》2019年第12期1561-1576,共16页分子植物（英文版）

基　　金：This work is supported by the NSFC(31730103,31825003,31522007);the National Key Basic Research Programme in China(2015CB158300);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB27040207);the International Partnership Program of Chinese Academy of Sciences(153D31KYSB20160074);the National Key Laboratory of Plant Molecular Genetics to E.W.

摘　　要：Symbiotic microorganisms improve nutrient uptake by plants.To initiate mutualistic symbiosis with arbus-cular mycorrhizal(AM)fungi,plants perceive Myc factors,including lipochitooligosaccharides(LCOs)and short-chain chitooligosaccharides(CO4/CO5),secreted by AM fungi.However,the molecular mechanism of Myc factor perception remains elusive.In this study,we identified a heteromer of LysM receptor-like kinases consisting of OsMYR1/OsLYK2 and OsCERK1 that mediates the perception of AM fungi in rice.CO4 directly binds to OsMYR1,promoting the dimerization and phosphorylation of this receptor complex.Compared with control plants,Osmyr1 and Oscerk1 mutant rice plants are less sensitive to Myc factors and show decreased AM colonization.We engineered transgenic rice by expressing chimeric receptors that respectively replaced the ectodomains of OsMYR1 and OsCERK1 with those from the homologous Nod factor receptors MtNFP and MtL YK3 of Medicago truncatula.Transgenic plants displayed increased cal-cium oscillations in response to Nod factors compared with control rice.Our study provides significant mechanistic insights into AM symbiotic signal perception in rice.Expression of chimeric Nod/Myc recep-tors achieves a potentially important step toward generating cereals that host nitrogen-fixing bacteria.

关 键 词：RICE ARBUSCULAR MYCORRHIZAL symbiosis Myc FACTORS Nod FACTORS LysM receptor-like KINASES 

分 类 号：R73[医药卫生—肿瘤]