Participation of the inositol 1,4,5-trisphosphategated calcium channel in the zona pellucida- and progesterone-induced acrosome reaction and calcium influx in human spermatozoa  

作　　者：Ying-Ya Li Yan-Ping Jia Li-Yan Duan Kun-Ming Li 

机构地区：[1]Center of Reproductive Medicine,Shanghai First Maternity and Infant Hospital,Tongji University School of Medicine,Shanghai 201204,China

出　　处：《Asian Journal of Andrology》2020年第2期192-199,共8页亚洲男性学杂志（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(grant No.81671521);the Natural Science Foundation of Shanghai(grant No.16ZR1427200);the Western Medical Guidance Project of Science and Technology Commission of Shanghai Municipality(grant No.17411968000);the Project of Three-year Action Plan to Promote Clinical Skills and Innovation Capabilities in Municipal Hospitals(grant No.16CR3069B).

摘　　要：The acrosome reaction is a prerequisite for fertilization,and its signaling pathway has been investigated for decades.Regardless of the type of inducers present,the acrosome reaction is ultimately mediated by the elevation of cytosolic calcium.Inositol 1,4,5-trisphosphate-gated calcium channels are important components of the acrosome reaction signaling pathway and have been confirmed by several researchers.In this study,we used a novel permeabilization tool BioPORTER?and first demonstrated its effectiveness in spermatozoa.The inositol 1,4,5-trisphosphate type-1 receptor antibody was introduced into spermatozoa by BioPORTER?and significantly reduced the calcium influx and acrosome reaction induced by progesterone,solubilized zona pellucida,and the calcium ionophore A23187.This finding indicates that the inositol 1,4,5-trisphosphate type-1 receptor antibody is a valid inositol 1,4,5-trisphosphate receptor inhibitor and provides evidence of inositol 1,4,5-trisphosphate-gated calcium channel involvement in the acrosome reaction in human spermatozoa.Moreover,we demonstrated that the transfer of 1,4,5-trisphosphate into spermatozoa induced acrosome reactions,which provides more reliable evidence for this process.In addition,by treating the spermatozoa with inositol 1,4,5-trisphosphate/BioPORTER?in the presence or absence of calcium in the culture medium,we showed that the opening of inositol 1,4,5-trisphosphate-gated calcium channels led to extracellular calcium influx.This particular extracellular calcium influx may be the major process of the final step of the acrosome reaction signaling pathway.

关 键 词：ACROSOME REACTION human SPERMATOZOA inositol 1 4 5-trisphosphate zona pellucida PROGESTERONE 

分 类 号：R697[医药卫生—泌尿科学]