高效液相色谱快速分析亚麻籽环肽条件研究  

Rapid analysis of cyclolinopeptides by HPLC

作　　者：许趁心 蔡子哲[1,2,3] 杜木香 刘雪 汪勇 王瑛[1,2,3] XU Chenxin;CAI Zizhe;DU Muxiang;LIU Xue;WANG Yong;WANG Ying(Department of Food Science and Engineering,Jinan University,Guangzhou 510632,China;Guangdong Engineering Technology Research Center for Cereal and Oil Byproduct Biorefinery,Guangzhou 510632,China;Guangdong Saskatchewan Oilseed Joint Laboratory,Guangzhou 510632,China)

机构地区：[1]暨南大学食品科学与工程系,广州510632 [2]广东省粮油副产物生物炼制工程技术研究中心,广州510632 [3]暨南大学-萨斯喀彻温大学“油料生物炼制与营养”联合实验室,广州510632

出　　处：《中国油脂》2020年第1期126-131,共6页China Oils and Fats

基　　金：“十三五”国家重点研发计划(2018YFD0401103-2);国家自然科学青年基金(31701525);广东省食品副产物增值加工产业技术创新联盟(2017B090907018)。

摘　　要：采用高效液相色谱法分析亚麻籽环肽组成及相对含量。以14种亚麻籽环肽色谱峰平均分离度和分析效率作为指标,对不同色谱柱、进样量、梯度洗脱初始体积分数、流速、乙腈体积分数上升速率进行优化,并利用HPLC-ESI-MS进行定性分析。结果表明,高效液相色谱最优条件为:采用Kinetex■色谱柱,水、乙腈为流动相,进样量5μL,梯度洗脱程序为乙腈体积分数从40%以5%/min升至90%,流速1 m L/min。最优条件下亚麻籽环肽14个色谱峰分离度较好,平均分离度为1. 65,分析时间为9. 09 min,分离效率为10. 86。优化后的色谱条件可对市售亚麻籽、亚麻籽油以及亚麻籽粕中环肽组成进行有效分析。The composition and relative content of cyclolinopeptides were analyzed by high performance liquid chromatography( HPLC). HPLC parameters( chromatographic column,injection volume,initial elution volume fraction,flow speed and increase rate of acetonitrile volume fraction) were optimized with the average degree of separation and separation efficiency of 14 kinds of cyclolinopeptide’peaks as an index. Cyclolinopeptides were qualitatively determined by HPLC-ESI-MS. The results showed that the optimal HPLC parameter were obtained as follows: Kinetex■ chromatographic column,water and acetonitrile as the mobile phase,flow rate 1 m L/min,injection volume 5 μL,elution programming of acetonitrile volume fraction increasing from 40% to 90% at 5%/min. 14 peaks of cyclolinopeptides had good separation,and the average degree of separation,analysis time and separation efficiency were 1. 65,9. 09 min and 10. 86,respectively. The optimal HPLC conditions could be applied in the determination of cyclolinopeptides composition of commercial flaxseed,flaxseed oil and flaxseed meal.

关 键 词：亚麻籽 亚麻籽环肽 高效液相色谱 平均分离度 分析时间 

分 类 号：O657.7+2[理学—分析化学] TS222+1[理学—化学]