分光光度法测定降血脂片中总皂苷含量的方法研究  

Determination of Total Saponins in Hypolipidemic Tablets by Spectrophotometry

作　　者：高宏[1] 黄婷[1] 徐慧[1] Gao Hong;Huang Ting;Xu Hui(Physics&Chemistry Test Center of Jiangsu Province,Nanjing 210042,China)

机构地区：[1]江苏省理化测试中心,江苏南京210042

出　　处：《中国野生植物资源》2020年第2期42-44,65,共4页Chinese Wild Plant Resources

摘　　要：建立一种简便、可靠的降血脂片中总皂苷含量的测定方法。以香草醛-高氯酸为显色体系,以人参皂苷为对照品,采用分光光度法测定降血脂片中总皂苷含量。比较分析了索氏提取法和超声波提取法对总皂苷提取分离效果的影响,并对大孔树脂填充高度进行了优化,最后对所建立的方法进行了重复性和加标回收率实验验证。结果表明,所测标准曲线的相关系数为0.9994,确立超声波提取法和3 cm大孔树脂填充高度为最佳实验条件,样品中总皂苷重复性相对标准偏差为1.8%,加标回收率为96.4%~99.0%,总皂苷测定重复性和回收率均良好。This article aims to establish a simple and reliable method for the determination of total saponin content in hypolipidemic tablets.The content of total saponins in hypolipidemic tablets was determined by spectrophotometry with vanillin-perchloric acid as the color system and ginsenoside as the reference substance.The effects of Soxhlet extraction and ultrasonic extraction on the extraction and separation of total saponins were compared and the filling height of macroporous resin was optimized.Finally,the established method was verified by repeatability and spike recovery.The results show that the correlation coefficient of the standard curve measured is 0.9994.The ultrasonic extraction method and the filling height of 3 cm macroporous resin are the best experimental conditions.The relative standard deviation of total saponins repeatability in the sample is 1.8%,and the recovery rate is 96.4~99.0%,the total saponins determination repeatability and recovery were good.

关 键 词：总皂苷 超声波 分光光度法 

分 类 号：TS-9[轻工技术与工程]