银杏提取物50通过调节自噬降低ROS水平保护高糖对血管内皮细胞的损伤作用  

Ginkgo biloba extract 50 protects vascular endothelial cells from hyperglycemia by lowering the ROS level via regulating autophagy

作　　者：姚媛 Yao Yuan(West China Fourth Hospital,Sichuan University,Chengdu 610011,China)

机构地区：[1]四川大学华西第四医院,成都610011

出　　处：《中国比较医学杂志》2020年第3期71-76,共6页Chinese Journal of Comparative Medicine

摘　　要：目的研究银杏叶提取物50在高糖环境下对血管内皮细胞(HUVECs)的保护作用。方法通过MTT法研究了GBE50对HUVECs细胞活性的影响,通过荧光反应法研究了谷胱甘肽的含量变化,通过流式细胞实验研究了HUVECs中活性氧含量和GBE50对细胞自噬的影响。结果 MTT实验结果显示GBE50可显著提高高糖环境下HUVECs细胞活性。在流式细胞实验中,高糖环境下HUVECs中ROS阳性细胞比率明显升高,而GBE50可显著降低ROS生成。并且GBE50可显著升高高糖环境下HUVECs中GSH含量,降低MDA阳性细胞比率。免疫荧光实验和蛋白印迹实验结果显示,GBE50可显著降低HUVECs中的自噬水平。结论 GBE50可通过降低细胞自噬水平、降低ROS产物含量降低高糖环境对血管内皮细胞细胞活性的影响。Objective To study the protective effect of Ginkgo biloba extract 50( GBE50) on vascular endothelial cells in a high glucose environment. Methods MTT assays were utilized to examine the HUVECs cell viability after the treatment of GBE50,changes in glutathione content were assessed by using the 7-amino-4-chloromethylcoumarin( CMAC)fluorescent stain technique,the effects of reactive oxygen species and GBE50 on autophagy were investigated by flow cytometry. Results MTT assay result showed that GBE50 significantly improved the viability of HUVECs in a high glucose environment. Flow cytometry showed that ROS-positive HUVECs were increased significantly in the high glucose environment,but GBE50 significantly reduced ROS production. GBE50 significantly increased the GSH content in HUVECs in the high glucose environment and decreased the ratio of MDA-positive cells. Immunofluorescence and western blot result showed that GBE50 significantly decreased the level of autophagy in HUVECs. Conclusions GBE50 reduces the effects of a high glucose environment on the activity of vascular endothelial cells by reducing autophagy and ROS production.

关 键 词：GBE50 HUVECS ROS 细胞自噬 

分 类 号：R-33[医药卫生]