基质辅助激光解吸电离飞行时间质谱检测实验动物病原菌效果初探  

Evaluation of the biotyper MALDI-TOF MS system for identification of pathogenic bacteria in laboratory animals

作　　者：邢进[1] 刘娜[2] 冯育芳[1] 张雪青 崔生辉[2] 贺争鸣[1] 赵德明[3] 岳秉飞[1] XING Jin;LIU Na;FENG Yufang;ZHANG Xueqing;CUI Shenghui;HE Zhengming;ZHAO Deming;YUE Bingfei(Institute for Laboratory Animal Resources,National Institutes for Food and Drug Control,Beijing 102629,China;Institute for Food and Cosmetics Control,National Institutes for Food and Drug Control,Beijing 100050;College of Veterinary Medicine,China Agricultural University,Beijing 100193)

机构地区：[1]中国食品药品检定研究院实验动物资源研究所,北京102629 [2]中国食品药品检定研究院食品化妆品检定所,北京100050 [3]中国农业大学动物医学院,北京100193

出　　处：《中国比较医学杂志》2020年第3期83-88,97,共7页Chinese Journal of Comparative Medicine

摘　　要：目的评价基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)检测实验动物病原菌的效果。方法使用德国Bruker Biotyper系统对40株标准菌株及384株实验动物分离菌进行鉴定。结果 Bruker Biotyper检测标准菌株和分离株的准确率分别为90. 0%和80. 0%。对鼠伤寒沙门氏菌(Salmonella typhimurium)、假结核耶尔森氏菌(Yersinia pseudotuberculosis)、志贺菌(Shigella spp.)、肺炎克雷伯杆菌(Klebsiella pneumoniae)、嗜肺巴斯德杆菌Heyl生物型(Rodentibacter heylii)和支气管鲍特杆菌(Bordetella bronchiseptica)的鉴定存在偏差。结论MALDI-TOF MS方法高效、准确率高,可用于实验动物病原菌的快速鉴定。Objective To evaluate matrix-assisted laser desorption ionization-time of flight mass spectrometry( MALDI-TOF MS) for detection of pathogenic bacteria in laboratory animals. Methods In total,40 standard strains and384 laboratory animal isolates were identified using the Bruker Biotyper system. Results The accuracy rates of Bruker Biotyper to detect standard strains and isolates were 90. 0% and 80. 0%, respectively. There were biases in the identification of Salmonella typhimurium,Yersinia pseudotuberculosis,Shigella spp.,Klebsiella pneumoniae,Pasteurella pneumotropica biotype Heyl,and Bordetella bronchiseptica. Conclusions The MALDI-TOF MS method is highly efficient and accurate. It can be used for rapid identification of pathogenic bacteria in laboratory animals.

关 键 词：基质辅助激光解吸电离飞行时间质谱 病原菌 细菌检测 实验动物 

分 类 号：R-33[医药卫生]