抗性糊精对高脂饮食诱导的小鼠肝脏脂肪沉积及AMPK通路的影响  

Effects of resistant dextrin on liver fat deposition and the AMPK signaling pathway in high-fat diet-fed mice

作　　者：胡秋月 何孙跃 鹿瑶 徐肖元 钮忆欣[1] 张洪梅[1] 苏青[1] Hu Qiuyue;He Sunyue;Lu Yao;Xu Xiaoyuan;Niu Yixin;Zhang Hongmei;Su Qing(Department of Endocrinology,Xinhua Hosipital,Shanghai JiaoTong University School of Medicine,Shanghai,200092,China)

机构地区：[1]上海交通大学医学院附属新华医院内分泌科,200092

出　　处：《中华内分泌代谢杂志》2020年第2期139-144,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金(81670743)。

摘　　要：目的观察抗性糊精对高脂饮食诱导的小鼠肝脏脂肪沉积及肝脏AMPK通路的影响。方法取4周龄雄性C57BL/6小鼠36只,随机分为3组,即正常对照组,高脂饮食组和高脂饮食+抗性糊精组(10 g·kg^-1·d^-1)。第12周末处死小鼠,留取血清测定三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、谷草转氨酶(AST)和谷丙转氨酶(ALT)水平;留取肝脏组织,测定TG含量,并予HE和油红O染色,观察肝细胞脂肪变性和肝脏脂肪沉积;实时荧光定量PCR检测肝脏组织脂肪酸合成相关基因SREBP1、ACC、SCD1 mRNA的表达水平,Western印迹检测肝脏pAMPK、SREBP1、Fasn、ACC蛋白的表达。结果与正常对照组相比,高脂饮食组小鼠体重增加更显著,空腹血糖(FBG)、血清TC、LDL-C、HDL-C、ALT水平均升高(P<0.01),血清AST也升高(P<0.05);肝脏油红O染色可见肝脏组织脂肪沉积明显,肝脏TG含量增加(P<0.01);SREBP1、ACC mRNA水平升高;pAMPK蛋白水平表达下降(P<0.05)。与高脂饮食组相比,抗性糊精组小鼠体重增加较少,血清TG、TC、LDL-C、HDL-C、ALT水平较低(P<0.01),FBG降低(P<0.05);肝脏油红O染色可见肝脏脂肪沉积减少,肝脏TG含量降低(P<0.01);SREBP1、ACC、SCD1 mRNA水平下降;pAMPK水平增加,Fasn蛋白水平下降(P<0.01)。结论抗性糊精可以改善高脂饮食诱导的小鼠肝脏脂肪沉积,这一作用可能与激活AMPK通路有关。Objective To study the effects of resistant dextrin(RD)on liver fat deposition in high-fat diet-fed(HFD)mice,and to further explore whether it can regulate the AMPK signaling pathway.Methods Thirty-six 4-week-old male C57BL/6 mice were randomly divided into three groups:normal control group(chow),high-fat diet group(HFD),and high-fat diet+resistant dextrin group(HFD+RD,10 g·kg^-1·d^-1).After 12 weeks of intervention,the liver tissues and serum samples were collected.Serum triglyceride(TG),total cholesterol(TC),low-density lipoprotein-cholesterol(LDL-C),high-density lipoprotein-cholesterol(HDL-C),aspartate aminotransferase(AST),alanine transaminase(ALT)levels and liver TG were measured.Liver tissue HE and oil red O staining were performed to observe hepatocyte steatosis and liver fat deposition.Quantitative real-time PCR was performed to detect the relative expression of fatty acid synthesis related genes SREBP1,ACC,SCD1 in the liver tissue,and Western blot was performed to detect relative protein levels of pAMPK,SREBP1,Fasn,and ACC in the liver.Results Compared with chow group,the body weight gain,fasting blood glucose(FBG),serum TC,LDL-C,HDL-C,and ALT levels were increased in HFD group(P<0.01),and serum AST level was also increased(P<0.05).Moreover,liver oil red O staining revealed that liver fat deposition was much more obvious in HFD group than that in chow group,and liver TG was also increased in HFD group(P<0.01).The mRNA levels of SREBP1 and ACC were increased in HFD group compared with that in chow group,and the protein level of pAMPK was reduced in HFD group(P<0.05).As compared with HFD group,the body weight gain,serum TG,TC,LDL-C,HDL-C and ALT levels were significantly reduced in RD group(P<0.01),and FBG level was also reduced(P<0.05).Moreover,RD treatment alleviated liver fat deposition and TG accumulation(P<0.01).The mRNA levels of SREBP1,ACC,and SCD1 were all reduced in RD group compared with HFD group.The protein level of pAMPK was increased,and the expression of Fasn was reduced with RD treatment(

关 键 词：抗性糊精 脂肪肝 肝脏脂肪沉积 腺苷一磷酸激活的蛋白激酶 

分 类 号：R575[医药卫生—消化系统]