基于网络药理学对沙利度胺治疗原发性肝癌作用机制的研究  

Mechanism research of thalidomide in treating primary liver cancer based on network pharmacology

作　　者：唐伟智 侯恩存 唐友明 郑景辉 吴竞 谢俊宇 TANG Weizhi;HOU Encun;TANG Youming;ZHENG Jinghui;WU Jing;XIE Junyu(Internal Medicine Oncology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi Zhuang Autonomous Region 530011,China;Gastroenterology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi Zhuang Autonomous Region 530011,China;Department of Internal Medicine Cardiovascular,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi Zhuang Autonomous Region 530011,China)

机构地区：[1]广州中医药大学附属瑞康医院肿瘤内科,广西壮族自治区南宁530011 [2]广州中医药大学附属瑞康医院消化内科,广西壮族自治区南宁530011 [3]广州中医药大学附属瑞康医院心血管内科,广西壮族自治区南宁530011

出　　处：《安徽医药》2020年第4期641-645,共5页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金项目(81360535);广西科学研究与技术开发计划项目(桂科攻1598013 5)。

摘　　要：目的在网络药理学的基础上探讨沙利度胺治疗原发性肝癌的作用机制。方法2018年6月至2018年12月,通过整合靶点预测网站及GeneCards、CTD、Liverome数据库的方法预测和筛选沙利度胺治疗原发性肝癌的作用靶点。通过DisGeNET数据库对作用靶点类型进行分类,并利用String数据库和Cytoscape软件绘制蛋白相互作用网络。通过DAVID数据库对沙利度胺潜在作用靶点进行基因本体论(Gene Ontology,GO)分析,通过KEGG Mapper进行代谢通路分析。最后通过系统对接网站(Systems Dock Web Site)对沙利度胺分子与作用靶点进行分子对接验证。结果通过预测和筛选得到沙利度胺治疗原发性肝癌的11个潜在作用靶点,主要通过调控癌症通路、核因子κB信号通路、肿瘤坏死因子信号通路及癌症蛋白聚糖通路发挥对原发性肝癌的治疗作用。结论沙利度胺治疗原发性肝癌体现了沙利度胺多靶点、多途径的治疗特点,为阐明其治疗原发性肝癌的作用机制提供了科学依据。Objective To explore the mechanism of Thalidomide in treating primary liver cancer basis on network pharmacology.Methods The targets of Thalidomide in treating primary liver cancer were collected by integrating the Target Prediction Website(SPTD),GeneCards,CTD,and Liverome databases from June 2018 to December 2018.Targets were classified through DisGeNET database.The protein protein interaction network was drawn through String database and Cytoscape software.Potential targets of Tha lidomide were analyzed by Gene Ontology in DAVID database.Metabolic pathways analysis was performed by KEGG Mapper.Final ly,Thalidomide molecule and potential targets were docked via Systems Dock Web Site to verify the molecular docking.Results Eleven potential targets for Thalidomide in treating primary liver cancer are predicted and screened.They play a therapeutic role in primary liver cancer by regulating pathways in cancer,NF kappa B signaling pathway,TNF signaling pathway and Proteoglycans in cancer.Conclusion Thalidomide in treating primary liver cancer embodies the multi target and multi channel characteristics of Thalidomide,which provides a scientific basis for elucidating its mechanism of action in treating primary liver cancer.

关 键 词：癌 肝细胞 沙利度胺 NF ΚB 肿瘤坏死因子类 蛋白聚糖类 网络药理学 癌症通路 

分 类 号：R735.7[医药卫生—肿瘤]