K RAS蛋白表达及基因状态在胃癌组织中的临床意义  

Clinical significance of K RAS protein expression and gene status in gastric carcinoma

作　　者：宗桂娟[1] 尹海兵[1] 李春笋[1] 陈旭东[1] 蔡南南 杨磊[2] 赵斌[1] 何松[1] ZONG Guijuan;YIN Haibing;LI Chunsun;CHEN Xudong;CAI Nannan;YANG Lei;ZHAO Bin;HE Song(Department of Pathology,Nantong Tumor Hospital,Nantong,Jiangsu 226361,China;Department of Oncology,Nantong Tumor Hospital,Nantong,Jiangsu 226361,China;Nantong University,Nantong,Jiangsu 226019,China)

机构地区：[1]南通市肿瘤医院病理科,江苏南通226361 [2]南通市肿瘤医院肿瘤内科,江苏南通226361 [3]南通大学杏林学院,江苏南通226019

出　　处：《安徽医药》2020年第4期705-708,共4页Anhui Medical and Pharmaceutical Journal

基　　金：南通市科技项目(HS2015003)。

摘　　要：目的分析胃癌病人的K RAS蛋白表达及基因状态与临床病理特征的关系,为胃癌病人的个性化治疗提供指导。方法选取2016年1月至2017年12月南通市肿瘤医院314例胃癌病人,分别采用免疫组织化学法及荧光定量PCR的方法分析K RAS蛋白表达水平和基因突变状态。结果314例胃癌组织中K RAS蛋白表达与胃癌病人的年龄(P=0.6485)、性别(P=0.5858)、肿瘤大小(P=0.7611)、脉管侵犯(P=0.5524)、淋巴结转移均无明显关系(P=0.5064),与组织学分级密切相关(P=0.0195);K RAS基因突变类型包括G12D、G13D、G12A、G12S、G13S;不同性别(P=0.4566)、年龄(P=0.5242)、组织分化之间(P=0.7474)的K RAS突变率差异无统计学意义;K RAS基因突变率与蛋白表达检出率差异有统计学意义(P=0.0377)。结论K RAS的蛋白表达程度与肿瘤的分化程度有关,K RAS基因在不同性别、年龄、组织分化之间的突变率差异无统计学意义,且K RAS基因突变率与蛋白表达不一致。Objective To analyze the relationship between K RAS protein expression and gene status and clinicopathological fea tures in patients with gastric carcinoma,and to provide guidance for personalized treatment of gastric carcinoma patients.Methods A total of 314 patients with gastric cancer in Nantong Cancer Hospital from January 2016 to December 2017 were selected.The ex pression level of K RAS protein and the state of gene mutation were analyzed by immunohistochemical method and fluorescent quantitative PCR.Results The expression of K RAS protein in 314 cases of gastric carcinoma had no significant relationship with age(P=0.6485),sex(P=0.5858),tumor size(P=0.7611),vascular invasion(P=0.5524)and lymph node metastasis(P=0.5064),which was closely related to histological grade(P=0.0195).K RAS gene mutation types include G12D,G13D,G12A,G12C,G13S;K RAS mutation rates were not significantly different between genders(P=0.4566),age(P=0.5242),and tissue differentiation(P=0.7474).K RAS gene mutation rate and protein expression detection rate were statistically different(P=0.0377).Conclusion The protein expression of K RAS is related to the degree of tumor differentiation.There is no significant difference in the mutation rate between K RAS gene in different gender,age and tissue differentiation,and the K RAS gene mutation rate is inconsistent with protein expression.

关 键 词：胃肿瘤 基因 ras 突变 免疫组织化学 荧光免疫测定 K RAS 临床病理特征 

分 类 号：R735.2[医药卫生—肿瘤]