黄连中盐酸小檗碱的提取纯化及抑菌活性研究  

Purification and antifungal activity of berberine hydrochloride in Rhizoma Coptidis

作　　者：屈欢 黄雪 王军节[1] 杨晨希 张琇[1,2] QU Huan;HUANG Xue;WANG Junjie;YANG Chenxi;ZHANG Xiu(College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China;Ningxia Key Laboratory for Development and Applications of Microbial Resources in Special Environment, Yinchuan 750021, China)

机构地区：[1]北方民族大学生物科学与工程学院,银川750021 [2]宁夏特殊生境微生物资源开发与利用重点实验室,银川750021

出　　处：《植物保护》2020年第2期96-100,共5页Plant Protection

基　　金：北方民族大学校级科研项目(2019XYZSK02);北方民族大学引进人员科研启动项目(4400302500);北方民族大学植物性农产品贮藏与加工重点实验室项目(2018NCPKL002)。

摘　　要：为了探究盐酸小檗碱在植物病原真菌方面的抑制作用,以黄连根粉为材料,采用酸水法提取和乙醇重结晶获得盐酸小檗碱纯品,采用生长速率法研究了盐酸小檗碱对常见植物病原菌的抑制活性。结果表明,盐酸小檗碱的提取率为3.3%,经高效液相色谱法测得其含量为93.56%;抑菌活性试验表明,盐酸小檗碱在100μg/mL时对玉米大斑病菌的抑制率达99.9%,EC50为8.20μg/mL,表现出比药剂对照噁霉灵和百菌清更高的抑制活性;进一步的试验表明,与噁霉灵及百菌清相比,盐酸小檗碱对玉米大斑病菌的抑制作用具有持效期长的特点。The aim of this study is to measure the antifungal activity of berberine hydrochloride.The root powder of Rhizoma Coptidis was used as material to obtain berberine hydrochloride,the purified product of which was successfully obtained through extraction by using acid-water method and recrystallization with ethanol,and the antifungal activity of berberine hydrochloride against phytopathogenic fungi was investigated using the mycelial growth rate method.The results showed that the extraction rate of berberine hydrochloride was 3.3%and its content was 93.56%measured by HPLC.The results of biological assay indicated that berberine hydrochloride exhibited an excellent antifungal activity against Exserohilum turcicum at the concentration of 100μg/mL with an inhibition rate of 99.9%and EC50 value of 8.20μg/mL,which were better than those of hymexazol and chlorothalonil,two commercially available agricultural fungicides.Compared with hymexazol and chlorothalonil,the inhibition effect of berberine hydrochloride on E. turcicum had a longer effective period.

关 键 词：盐酸小檗碱 提取纯化 含量测定 抑菌活性 

分 类 号：S482.292[农业科学—农药学]