近平滑假丝酵母ATCC 7330对不同取代基乙酮类底物的催化效果  

Catalytic Effect of Candida parapsilosis ATCC 7330 on Ethyl Ketone Substrates with Different Substituted Groups

作　　者：刘云[1] 王伟[1] 郭金玲[1] 任立伟 罗华军[1] 余华顺[2] 戴秋红[2] 姚鹃[2] 龚大春[1] LIU Yun;WANG Wei;GUO Jinling;REN Liwei;LUO Huajun;YU Huashun;DAI Qiuhong;YAO Juan;GONG Dachun(Hubei Engineering Technology Research Center for Biological Jiaosu(HETRC-BJ),China Three Gorges University,Yichang 443002,China;Enzyme Preparation Department,Angel Yeast Co.,Ltd.,Yichang 443002,China)

机构地区：[1]三峡大学,湖北省生物酵素工程技术研究中心,湖北宜昌443002 [2]安琪酵母股份有限公司酶制剂事业部,湖北宜昌443002

出　　处：《化学与生物工程》2020年第2期17-21,共5页Chemistry & Bioengineering

基　　金：国家自然科学基金项目(21776162);宜昌市科技创新项目(A15-301-b04);三峡大学学位论文培优基金项目(2019SSPY155)。

摘　　要：通过对接模拟和催化转化实验研究了近平滑假丝酵母ATCC 7330对10种不同取代基乙酮类底物的催化效果。结果表明:近平滑假丝酵母ATCC 7330整细胞催化对芳环取代乙酮类底物的光学选择性优于脂环取代乙酮类底物,对氨基苯乙酮的光学选择性最高,与模拟对接预测的结合能大小一致;从催化剂周转数来看,近平滑假丝酵母ATCC 7330整细胞对10种不同取代基乙酮类底物的催化能力大小依次为对氯苯乙酮=邻氯苯乙酮>1-萘乙酮>对氨基苯乙酮>环戊基乙酮>环己基乙酮>邻氨基苯乙酮>1-(1H-茚-4-基)乙酮>苯乙酮>环丙基乙酮,与对接模拟预测有一定差距。说明近平滑假丝酵母ATCC 7330整细胞的催化能力除了与结合能有关外,还与细胞的渗透性、反应溶剂等因素有关。We studied the catalytic effect of Candida parapsilosis ATCC 7330 on ten ethyl ketone substrates with different substituted groups by CDOCKER simulation and catalytic conversion experiments.The results show that the optical selectivity of Candida parapsilosis ATCC 7330 whole-cell catalysis for aryl-substituted ethyl ketone is better than that for alicyclic-substituted ethyl ketone,and p-aminoacetophenone has the highest optical selectivity,which is consistent with the predicted binding energy of CDOCKER simulation.From the perspective of the turnover number of catalyst,the catalytic capability of Candida parapsilosis ATCC 7330 whole-cell to ten ethyl ketone substrates with different substituted groups is in the following sequence:p-chloroacetophenone=o-chloroacetophenone>1-acetonaphthone>p-aminoacetophenone>cyclopentyl-ethanone>cyclohexyl-ethanone>o-aminoacetophenone>1-(1H-indene-4-yl)ethanone>acetophenone>cyclopropyl-ethanone,which is different from the prediction of CDOCKER simulation to a certain extent.The catalytic capability of Candida parapsilosis ATCC 7330 whole-cell is related not only to the binding energy,but also to the cell permeability,reaction solvent,and other factors.

关 键 词：近平滑假丝酵母ATCC 7330 乙酮类底物 底物适应性 催化效果 

分 类 号：TQ920[轻工技术与工程—发酵工程] Q814[生物学—生物工程]