Enhanced Production of Natural Carotenoids from Genetically Engineered Rhodobacter sphaeroides Overexpressing CrtA  

作　　者：Zhiping ZHAO Jiahui DU Hongfan CHEN Lili JI Jiamin ZHANG Wei WANG 

机构地区：[1]Meat Processing Key Laboratory of Sichuan Province,Chengdu University,Chengdu 610106,China

出　　处：《Agricultural Biotechnology》2020年第2期1-4,共4页农业生物技术（英文版）

基　　金：Supported by the Project of Sichuan Science and Technology Department(2019YJ0673);National Modern Agriculture Industry System/Sichuan Live Pig Innovation Team(SCSZTD-3-007)。

摘　　要：Carotenoids act as precursors of vitamin A,antioxidants,enhancers of immunity,and are thus widely used in food and pharmaceutical industry.Microbial fermentation is one of the most important solutions for production of natural carotenoids.Rhodobacter sphaeroides is one of most promising bacteria employed for large scale production of carotenoids.In the present study,crtA located in the carotenoids biosynthesis pathway in R.sphaeroides was amplified by PCR.The overexpression vector pRKcrtA was constructed and subsequently transferred into R.sphaeroides,producing the genetically engineered strain R.sphaeroides 2.4.1/pRKcrtA overexpressing crtA.The carotenoid production from the genetically engineered strain was significantly increased.Fermentation procedure was optimized for further enhanced carotenoids production.Carotenoids act as precursors of vitamin A,antioxidants,enhancers of immunity,and are thus widely used in food and pharmaceutical industry. Microbial fermentation is one of the most important solutions for production of natural carotenoids. Rhodobacter sphaeroides is one of most promising bacteria employed for large scale production of carotenoids. In the present study,crtA located in the carotenoids biosynthesis pathway in R. sphaeroides was amplified by PCR. The overexpression vector pRKcrtA was constructed and subsequently transferred into R. sphaeroides,producing the genetically engineered strain R. sphaeroides 2. 4. 1/pRKcrtA overexpressing crtA. The carotenoid production from the genetically engineered strain was significantly increased. Fermentation procedure was optimized for further enhanced carotenoids production.

关 键 词：Carotenoids CRTA RHODOBACTER sphaeroids FERMENTATION OVEREXPRESSION Optimization 

分 类 号：Q78[生物学—分子生物学]