In-vitro Synthesis and Activity Identification of Antimicrobial Peptide from Aspergillus granifolium  

作　　者：Huihui CAO Lei WANG Yan WANG Yanhua YAN Xiaonan SONG Yu ZHOU 

机构地区：[1]Tangshan Food and Drug Comprehensive Testing Center,Tangshan 063000,China [2]Hebei Agricultural Products Quality and Safety Testing Innovation Center,Tangshan 063000,China [3]Tangshan Institute of Industrial Technology for Functional Agricultural Products,Tangshan 063000,China

出　　处：《Agricultural Biotechnology》2020年第2期35-38,共4页农业生物技术（英文版）

基　　金：Supported by Tangshan Talent Funding Project in Hebei Province(A201905010)。

摘　　要：[Objectives] This study was conducted to investigate the control effect of antimicrobial peptides to peanut aflatoxin contamination and achieve the purpose of biological control of peanut aflatoxin.[Methods]Bioinformatics methods were applied to screen antimicrobial peptides with strong antifungal activity in the antimicrobial peptide database and analyze their secondary structures and bacteriostatic characteristics,and the candidated peptide was then artificially synthesized,expressed and purified and detected for in-vitro activity.[Results] The antimicrobial peptide(AFP1) which was screened from Aspergillus granifolium exhibited strong antimicrobial activity against Aspergillus flavus.The inhibition rate of crude AFP1 at 3 mg/ml reached 58% for gram-positive bacteria,52% for gram-negative bacteria,and 62% for A.flavus.[Conclusions]This study provides a new approach for the prevention and control of various plant diseases including peanut disease,and a new idea for the biological prevention and control of plant diseases.[Objectives] This study was conducted to investigate the control effect of antimicrobial peptides to peanut aflatoxin contamination and achieve the purpose of biological control of peanut aflatoxin. [Methods]Bioinformatics methods were applied to screen antimicrobial peptides with strong antifungal activity in the antimicrobial peptide database and analyze their secondary structures and bacteriostatic characteristics,and the candidated peptide was then artificially synthesized,expressed and purified and detected for in-vitro activity. [Results] The antimicrobial peptide( AFP1) which was screened from Aspergillus granifolium exhibited strong antimicrobial activity against Aspergillus flavus. The inhibition rate of crude AFP1 at 3 mg/ml reached 58% for gram-positive bacteria,52% for gram-negative bacteria,and 62% for A. flavus. [Conclusions]This study provides a new approach for the prevention and control of various plant diseases including peanut disease,and a new idea for the biological prevention and control of plant diseases.

关 键 词：PEANUT ANTIMICROBIAL peptide ASPERGILLUS granifolium ANTI-FUNGAL ACTIVITY BACTERIOSTATIC ACTIVITY detection 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S432.4[农业科学—植物保护]