Tamarix chinensis Lour inhibits chronic ethanol-induced liver injury in mice  

作　　者：Zhi-Dan Wang Yu Zhang Yi-Dan Dai Ke Ren Chen Han Heng-Xiao Wang Shuang-Qin Yi 

机构地区：[1]Laboratory of Functional Morphology,Graduate School of Human Health Sciences,Tokyo Metropolitan University,Tokyo 116-8551,Japan [2]Institute of Basic Medicine,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250062,Shandong Province,China

出　　处：《World Journal of Gastroenterology》2020年第12期1286-1297,共12页世界胃肠病学杂志（英文版）

基　　金：the Innovation Project of Shandong Academy of Medical Science;the Science and Technology Major Project of Shandong province,No.2015ZDJS03002.

摘　　要：BACKGROUND Tamarix chinensis Lour(TCL)is a shrub that usually grows in arid or semiarid desert areas and saline-alkali fields.It is a traditional Chinese herbal medicine with hepatoprotective,antioxidant,antibacterial,and antitumor activities.AIM To investigate the possible protective effects of TCL against liver injury induced by chronic ethanol intake.METHODS C57BL/6J male mice were fed a Lieber-DeCarli lipid diet containing alcohol and received(by gavage)a water-alcohol extract(80%)of TCL(100 and 200 mg/kg BW)or distilled water for 4 wk.After euthanasia,liver tissues were observed histologically with hematoxylin and eosin staining and Oil red O staining,and the levels of alanine aminotransferase,aspartate transaminase,hepatic lipids,reactive oxygen species,malondialdehyde,and superoxide dismutase were measured.In addition,expression of the NOD-like receptor family,pyrin domain-containing 3(NLRP3)inflammasome and downstream proinflammatory cytokines were determined.RESULTS Compared with the ethanol group,mice in the TCL-treated group(200 mg/kg)had significantly lower serum levels of alanine aminotransferase(mean,34.1 IU/L vs 45.3 IU/L,P<0.01)and aspartate transaminase(mean,89.6 IU/L vs 115.7 IU/L,P<0.01),as well as marked reduction of hepatic tissue reactive oxygen species(decreased by 27.5%,P<0.01)and malondialdehyde(decreased by 76.6%,P<0.01)levels,with a significant increase of superoxide dismutase(Increased by 73.2%,P<0.01).Expression of the NLRP3 inflammasome and its downstream cytokines[interleukin(IL)-1β,tumor necrosis factor-α,and IL-6],and recruitment of natural killer T cells to the liver,were reduced in the TCLtreated incubation with a Lieber-DeCaril ethanol lipid diet group.CONCLUSION These findings suggest that a TCL extract(200 mg/kg)protects against chronic ethanol-induced liver injury,probably by inhibiting the NLRP3-caspase-1-IL-1βsignaling pathway and suppressing oxidative stress.BACKGROUND Tamarix chinensis Lour(TCL) is a shrub that usually grows in arid or semiarid desert areas and saline-alkali fields. It is a traditional Chinese herbal medicine with hepatoprotective, antioxidant, antibacterial, and antitumor activities.AIM To investigate the possible protective effects of TCL against liver injury induced by chronic ethanol intake.METHODS C57 BL/6 J male mice were fed a Lieber-DeCarli lipid diet containing alcohol and received(by gavage) a water-alcohol extract(80%) of TCL(100 and 200 mg/kg BW) or distilled water for 4 wk. After euthanasia, liver tissues were observed histologically with hematoxylin and eosin staining and Oil red O staining, and the levels of alanine aminotransferase, aspartate transaminase, hepatic lipids,reactive oxygen species, malondialdehyde, and superoxide dismutase were measured. In addition, expression of the NOD-like receptor family, pyrin domain-containing 3(NLRP3) inflammasome and downstream proinflammatory cytokines were determined.RESULTS Compared with the ethanol group, mice in the TCL-treated group(200 mg/kg)had significantly lower serum levels of alanine aminotransferase(mean, 34.1 IU/L vs 45.3 IU/L, P < 0.01) and aspartate transaminase(mean, 89.6 IU/L vs115.7 IU/L, P < 0.01), as well as marked reduction of hepatic tissue reactive oxygen species(decreased by 27.5%, P < 0.01) and malondialdehyde(decreased by 76.6%, P < 0.01) levels, with a significant increase of superoxide dismutase (Increased by 73.2%, P < 0.01). Expression of the NLRP3 inflammasome and its downstream cytokines [interleukin(IL)-1β, tumor necrosis factor-α, and IL-6],and recruitment of natural killer T cells to the liver, were reduced in the TCLtreated incubation with a Lieber-DeCaril ethanol lipid diet group.CONCLUSION These findings suggest that a TCL extract(200 mg/kg) protects against chronic ethanol-induced liver injury, probably by inhibiting the NLRP3-caspase-1-IL-1βsignaling pathway and suppressing oxidative stress.

关 键 词：TAMARIX chinensis Lour ALCOHOLIC LIVER disease Ethanol-induced LIVER injury NLRP3 INFLAMMASOME Oxidative stress Natural KILLER T cells 

分 类 号：R285.5[医药卫生—中药学]