魔芋寡糖的膜分离及产物组成分析  

Membrane Separation and Product Composition Analysis of Konjac Oligosaccharides

作　　者：唐湘华[1,2,3] 杨云娟 顾天坤[3] 慕跃林 吴倩 黄遵锡[1,2,3] TANG Xiang-hua;YANG Yun-juan;GU Tian-kun;MU Yue-lin;WU Qian;HUANG Zun-xi(Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education,Yunnan Normal University,Kunming 650500,China;Enzymatic Engineering Technology Research Center of Yunnan Province,Kunming 650500,China;School of Life Sciences,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学生物能源持续开发利用教育部工程研究中心,云南昆明650500 [2]云南省酶工程技术研究中心,云南昆明650500 [3]云南师范大学生命科学学院,云南昆明650500

出　　处：《食品工业科技》2020年第8期21-25,共5页Science and Technology of Food Industry

基　　金：国家重点研发计划(2017YFB0308401)。

摘　　要：本文采用高速离心、无机陶瓷微孔滤膜、超滤纳滤对250 g/L(w/v)的高浓度魔芋精粉的酶解液进行分离、过滤、浓缩、制备魔芋寡糖,分析了甘露聚糖酶水解催化高浓度魔芋粉形成的具有一定粘性的寡糖溶液,对过滤通量、寡糖的回收率和寡糖产物组成的影响。结果表明,150 g/L的寡糖混合物通过超滤膜过滤,寡糖回收率能达到86%以上;组合纳滤膜能使寡糖混合物进行有效浓缩,可以去除溶液中的金属离子、有机酸小分子和溶剂水,提高寡糖溶质纯度。100~5000 Da的魔芋寡糖混合物通过基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)分析发现,寡糖的构成比例中三糖为最高单一组分,含量为21.5%,二糖~十四糖的总含量达到90%,十五糖-二十糖的含量为9%。因此,采用膜过滤技术用于魔芋寡糖分离、提纯和浓缩工艺是可行的。Centrifuge,micro porous filter membrane,and ultrafiltration-nanofiltration were used to separate,filter,and concentrate the enzymatic hydrolysis solution of konjac for preparing konjac oligosaccharides.Soluble glycan filtrates of 150 g/L filtered through the ultrafiltration membrane and the oligosaccharides recovery rate could reach over 86%.The filtration flux of the nanofiltration membrane decreased with the concentration of soluble glycan increasing.The matrix-assisted laser desorption ionization-time of flight mass spectrometer(MALDI-TOF-MS)used to analyze 100~5000 Da konjac oligosaccharides mixtures.The results showed that the content of trisaccharide was the highest single component with 21.5%,the total content of disaccharides to(C6H10O5)14 reached 90%,and(C6H10 O5)15^(C6H10 O5)20 content was 9%.It was feasible to use membrane filtration technology in the separation,purification and concentration of konjac oligosaccharides.

关 键 词：魔芋寡糖 膜分离 过滤通量 基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS) 产物组成分析 

分 类 号：TS201.4[轻工技术与工程—食品科学]