南京^192Ir放射事故患者照后第4年细胞遗传学随访  

Cytogenetic analysis of the victim followed-up at 4 year after 192Ir radiation accident in Nanjing

作　　者：陆雪[1] 赵骅[1] 王进[2] 李爽[1] 田雪蕾[1] 王福如[2] 田梅[1] 余宁乐[2] 刘青杰[1] Lu Xue;Zhao Hua;Wang Jin;Li Shuang;Tian Xuelei;Wang Furu;Tian Mei;Yu Ningle;Liu Qingjie(National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,China CDC Key Laboratory of Radiological Protection and Nuclear Emergency,WHO Collaborating Center for Radiation and Health(No.CHN-145),Beijing 100088,China;Department of Radiation Protection,Jiangsu Provincial Center of Disease Control and Prevention,Nanjing 210009,China)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,世界卫生组织辐射防护与健康合作中心,北京100088 [2]江苏省疾病预防控制中心放射防护所,南京210009

出　　处：《中华放射医学与防护杂志》2020年第3期183-188,共6页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的用5种细胞遗传学方法对南京192Ir源放射事故患者进行照后第4年随访,筛选回顾性剂量估算指标,为探明电离辐射远后效应提供依据。方法采集事故患者外周血,进行非稳定性染色体畸变(双着丝粒+着丝粒环)、微核和核质桥分析,应用荧光原位杂交技术及G显带方法检测染色体易位,并估算生物剂量。结果荧光原位杂交技术及G显带方法检测染色体易位估算的剂量分别为1.45和1.21 Gy,与事故后短期内估算的生物剂量相近;非稳定性染色体畸变、微核、核质桥估算的剂量分别为0.56、0.45、0.41 Gy,低于事故后短期内估算的生物剂量,其修正系数与时间的变化规律符合幂函数模型。结论荧光原位杂交技术及G显带方法检测染色体易位估算方法,适用于回顾性剂量估算,应用非稳定性染色体畸变进行回顾性剂量估算需用修正系数进行校正。Objective To screen the indicators of retrospective dose estimation,based on 5 cytogenetic methods to assess the victim followed-up at 4 year after ^192Ir radiation accident in Nanjing.Methods The chromosome aberration(dic+r)assay,cytokinesis block micronucleus(MN)and nucleoplasmic bridge(NPB)assay,fluorescence in situ hybridization(FISH)-based and G banding-based translocation analysis were used to retrospective biological dose estimation.Results The estimated doses of FISH-based and G banding-based analysis were 1.45 and 1.21 Gy respectively,which was similar to the biological dose estimated short time after the accident.However,the estimated doses by chromosome aberration,micronucleus and nucleoplasmic bridge method were 0.56,0.45 and 0.41 Gy respectively,which were lower than the corresponding biodose.Correction factors were used to adjust the biodose.Conclusions In the 4th years after exposure,the estimated biological doses by FISH-based and G banding-based translocation were consistent with the biodose.Therefore,the two methods were suitable for retrospective dose estimation,while correction factors should be considered in chromosome aberration method for retrospective dose estimation.

关 键 词：染色体畸变 荧光原位杂交 G显带 生物剂量估算 修正系数 

分 类 号：R394[医药卫生—医学遗传学]