清燥救肺汤对肺癌磷酸戊糖能量代谢途径的关键酶G6PD活性及其调控因子的影响  

Effect of Qingzao Jiufei Tang on Enzymatic Activity and Regulatory Factor of Key Enzyme G6PD in Pentose Phosphate Energy Metabolism Pathway in Lung Cancer

作　　者：陈江涛 余功 谢斌[1] CHEN Jiang-tao;YU Gong;XIE Bin(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学,南昌330004

出　　处：《中国实验方剂学杂志》2020年第4期59-63,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81660729);江西省卫计委中医药课题项目(2017A232)。

摘　　要：目的:观察清燥救肺汤对肺癌磷酸戊糖能量代谢途径6-磷酸葡萄糖脱氢酶(G6PD)活性及其调控因子的影响,探讨其机制。方法:50只雄性C57BL6J小鼠,通过随机分组,分为模型组、环磷酰胺组、清燥救肺汤高、中、低剂量组,每组10只。右腋下注射Lewis肺癌细胞建立肺癌荷瘤模型,清燥救肺汤高、中、低剂量组分别以11,5.5,2.8 g·kg^-1·d^-1造模前2周开始灌胃给药,环磷酰胺组以50 mg·kg^-1·(2 d)-1腹腔注射给药,模型组以等体积生理盐水灌胃给药,接种后继续给药2周后处死各组小鼠并取瘤组织,酶联免疫吸附测定(ELISA)检测G6PD活性及小鼠活性氧(ROS)含量;实时荧光定量聚合酶链式反应(Real-time PCR)检测癌组织中还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶亚基单位gp91phox和p22phox mRNA的表达。结果:与模型组比较,清燥救肺汤高、中、低剂量组及环磷酰胺组G6PD活性明显降低(P<0.05,P<0.01);清燥救肺汤高、中、低剂量组及环磷酰胺组ROS含量,NADPH氧化酶亚基单位gp91phox,p22phox mRNA表达显著降低(P<0.01)。结论:清燥救肺汤可能通过抑制NADPH氧化酶表达,减少ROS含量,抑制G6PD的表达,发挥抑制肺癌细胞能量代谢及细胞增殖的功效。Objective:To discuss the effect of Qingzao Jiufei Tang on enzymatic activity and regulatory factor of glucose 6-phosphatedehydrogenase(G6 PD)in pentose phosphate energy metabolism pathway in lung cancer.Method:Fifty male C57 BL6 J mice were randomly divided into five groups.Animal models were induced through axillary injection with Lewis cells.The Qingzao Jiufei Tang group was given drugs(11,5.5,2.8 g·kg^-1·d^-1)two weeks before modeling,the cyclophosphamide(CTX)group was intraperitoneally injected with CTX(50 mg·kg^-1),and the model group was intraperitoneally injected with the same volume of saline after molding.At 14 d after modeling,the mice were sacrificed,and the tumor tissues were collected.The enzymatic activity of G6 PD,content of reactive oxygen species(ROS)were detected by enzyme-linked immunosorbent assay(ELISA)method.Expressions of gp91 phox and p22 phox mRNA were detected by real-time fluorescent quantitative polymerase chain reaction(Real-time PCR)method.Result:Compared with the model group,the enzymatic activity of G6 PD in high-dose group,medium-dose group and low-dose group were reduced obviously(P<0.05,P<0.01).Content of ROS,mRNA expressions of gp91 phox and p22 phox in high-dose group,medium-dose group and low-dose group were reduced obviously(P<0.01).Conclusion:Qingzao Jiufei Tang may inhibit the expression of G6 PD by inhibiting the expression of gp91 phox,p22 phox oxidase,and then reduce content of ROS,so as to reduce the energy metabolism and hyperplasia of lung cancer cells.

关 键 词：清燥救肺汤 磷酸戊糖 调控因子 6-磷酸葡萄糖脱氢酶 小鼠活性氧 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]