纤维素酶-乙醇结合法提取人参总皂苷提取工艺的研究  

Cellulase-ethanol combination extraction of total ginsenosides from ginseng

作　　者：刘长姣[1] 杨柳[1] 陈宇飞[1] LIU Chang-jiao;YANG Liu;CHEN Yu-fei(Jilin Province Key Laboratory of Grain and Oil Processing,Jilin Business and Technology College,Changchun 130507)

机构地区：[1]吉林工商学院粮油食品深加工吉林省高校重点实验室,长春130507

出　　处：《中国食品添加剂》2020年第3期56-61,共6页China Food Additives

基　　金：吉林省科技发展计划项目(20170311022YY)。

摘　　要：利用纤维素酶-乙醇结合法提取人参总皂苷。通过单因素试验考察提取体系pH、纤维素酶添加量、乙醇浓度、酶解温度、料液比、提取时间和提取次数对人参总皂苷提取率的影响。利用正交试验设计得到最优提取条件:提取温度为50℃、提取次数为2次,pH为5.0、乙醇浓度为40%,酶添加量为1.5%、固液比为1∶12、提取时间为2h。在最优条件下,纤维素酶-乙醇结合法提取人参总皂苷的得率为2.73%。对比试验证明,纤维素酶-乙醇结合法提取的人参总皂苷提取率高于参考条件下回流提取法和浸提法。结果证明纤维素酶-乙醇结合法可以得到较高的人参总皂苷提取率。Ginsenosides were extracted by cellulase-ethanol combination extraction from ginseng. The effects of cellulase enzyme dosage, ethanol concentration, pH, extraction time, extraction temperature and solid liquid ratio on the yields of ginsenosides were studied using single factor method. The optimum extraction process was optimized by orthogonal test. The optimal extraction conditions were : extraction temperature 50℃, extract twice, pH 5.0, ethanol concentration 40%, enzyme dosage 1.5%, solid-liquid ratio 1∶12, extraction time 2 h. Under the above conditions, total ginsenosides extraction yield was 2.73%. The method has a higher yield of ginsenosides than heat reflux extraction and leaching method at the reference extraction conditions. The conclusion is that ginsenosides extraction yield can be improved by cellulase-ethanol extraction method.

关 键 词：人参 人参皂苷 纤维素酶-乙醇结合提取法 

分 类 号：TS201.1[轻工技术与工程—食品科学] TS202.3[轻工技术与工程—食品科学与工程]