常压室温等离子体诱变选育核黄素高产突变株  

Mutagenesis of Bacillus subtilis using the atmospheric and room temperature plasma technology for improved riboflavin production

作　　者：祝金山 吴烨飞 陆建卫 ZHU Jinshan;WU Yefei;LU Jianwei(Zhejiang Qianjiang Biochemistry Co.,Ltd.,Jiaxing 314400,China)

机构地区：[1]浙江钱江生物化学股份有限公司,浙江嘉兴314400

出　　处：《发酵科技通讯》2020年第1期58-62,共5页Bulletin of Fermentation Science and Technology

摘　　要：以核黄素生产菌枯草芽孢杆菌QJVB-1为出发菌株,采用常压室温等离子体(ARTP)诱变技术,通过平板初筛,摇瓶发酵复筛,得到一株核黄素产量高、遗传性状稳定的突变株QJVB-2-91。摇瓶发酵结果表明:突变株核黄素最高产量达到9447 mg/L,较出发菌株提高16.8%,发酵周期缩短6 h。5 L罐发酵结果表明:突变株核黄素最高产量达22001 mg/L,较出发菌株提高15.4%,发酵周期缩短6 h。经5次连续传代实验,突变株QJVB-2-91核黄素合成水平没有下降,可见突变株QJVB-2-91较出发菌株发酵水平显著提高,遗传性状稳定,发酵周期缩短,为产业化水平提高提供了坚实基础。Riboflavin is a type of B vitamin,which plays essential physiological roles in human bodies and animals.It is used as medicine and dietary supplement widely.To improve the productivity of riboflavin,the atmospheric and room temperature plasma(ARTP)mutation breeding technology was used to mutagen the riboflavin producing strain Bacillus subtilis.The riboflavin yield of the selected mutant reached 9447 mg/L,which was 16.8%higher than the parent strain in flask fermentation,while the fermentation period was shortened by 6 hours.The fermentation was further evaluated in a 5 L fermentation.The riboflavin yield reached 22001 mg/L,which was 15.4%higher than the parent strain.Moreover,the fermentation period of the mutant was 6 hours,less than the parent strain.This fermentation was tested for 5 rounds with the obtained mutant and the yield was constantly maintained at the high level.It is the first report on the application of ARTP technology in the breeding of riboflavin producing bacteria.Due to higher riboflavin yield and shorted fermentation time,the selected mutant is suitable for industrial application.

关 键 词：核黄素 常压室温 等离子体诱变 菌种选育 

分 类 号：TQ92[轻工技术与工程—发酵工程]