Constructing boronate-bridged core-satellite gold nanoassembly and its application in high sensitive colorimetric detection of benzoyl peroxide residues in food matrices  

作　　者：Ting Li Jiefang Sun Jinyuan Liu Baolei Dong Huachao Zhao Xiaoting Qiao Wencong Shan Jing Zhang Bing Shao 

机构地区：[1]School of Public Health,Capital Medical University,Beijing 100069,China [2]Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing Center for Disease Prevention and Control,Beijing 100013,China [3]Beijing Advanced Innovation Center for Food Nutrition and Human Health,China Agricultural University,Beijing 100193,China [4]College of Chemical Engineering and Materials Science,Tianjin University of Science and Technology,Tianjin 300457,China

出　　处：《Chinese Chemical Letters》2020年第2期439-442,共4页中国化学快报（英文版）

基　　金：financial support from the National Natural Science Foundation of China(Nos.U1736201,21677019 and 41403021);Capital Health Research and Development of Special(No.2018-4-1014);the Key Research and Development Program of Beijing(No.D171100008317001)。

摘　　要：Here,a new designed core/satellite gold nanoprobe was developed for detecting trace mount of benzoyl peroxide(BPO) based on its deboronation.This gold nanoassembly(the BE-Au NPs_(12/65)) wa s constructed via borate ester formation between large 4-mercaptophenylboronic acid(MPBA) modified Au NPs(the MPBAAu NPs_(65),as cores) and small dopamine modified AuNPs(the D PA-AuNPs_(12),as satellites).Particularly,upon addition of BPO,it would trigger the deboronation for the BE-AuNPs_(12/65) probes accompanying with distinct color changes from blue,purple to wine red,which implied the disassembly of the core/satellite nanostructure after the breakage of carbon to boron chemical bond.By measuring the absorbance ratio at 665 nm and 545 nm,quantification of BPO was achieved in the range of 10.0-100.0 nmol/L,which could also be easily observed by naked eyes.The nanoprobe utilized a boronate deprotection mechanism and the LSPR properties of Au NPs to provide high selectivity for detecting BPO over similar ROS/RNS with the limit of detection as low as 7.2 nmol/L.The practical applicability of this assay was verified through successful determining BPO in flour samples,which demonstrated its great potentials in food safety field.Here,a new designed core/satellite gold nanoprobe was developed for detecting trace mount of benzoyl peroxide（BPO） based on its deboronation.This gold nanoassembly(the BE-Au NPs12/65) wa s constructed via borate ester formation between large 4-mercaptophenylboronic acid（MPBA） modified Au NPs(the MPBAAu NPs65,as cores) and small dopamine modified AuNPs(the D PA-AuNPs12,as satellites).Particularly,upon addition of BPO,it would trigger the deboronation for the BE-AuNPs12/65 probes accompanying with distinct color changes from blue,purple to wine red,which implied the disassembly of the core/satellite nanostructure after the breakage of carbon to boron chemical bond.By measuring the absorbance ratio at 665 nm and 545 nm,quantification of BPO was achieved in the range of 10.0-100.0 nmol/L,which could also be easily observed by naked eyes.The nanoprobe utilized a boronate deprotection mechanism and the LSPR properties of Au NPs to provide high selectivity for detecting BPO over similar ROS/RNS with the limit of detection as low as 7.2 nmol/L.The practical applicability of this assay was verified through successful determining BPO in flour samples,which demonstrated its great potentials in food safety field.

关 键 词：BENZOYL PEROXIDE COLORIMETRIC detection GOLD nanoassembly Deboronation FOOD safety 

分 类 号：TS207.3[轻工技术与工程—食品科学] TB383.1[轻工技术与工程—食品科学与工程]