三株海南岛野生灵芝的鉴定、多糖组成及其抗氧化活性研究  

Identification,polysaccharide composition and antioxidant activity of three wild Ganoderma strains in Hainan Island

作　　者：金鑫[1] 熊川[1] 李萍[1] 李强 陈祖琴[1] 张璐 黄文丽[1] JIN Xin;XIONG Chuan;LI Ping;LI Qiang;CHEN Zu-qin;ZHANG Lu;HUANG Wen-li(Biotechnology and Nuclear Technology Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610061,China;College of Pharmacy and Biological Engineering,Chengdu University,Chengdu 610106,China)

机构地区：[1]四川省农业科学院生物技术核技术研究所,成都610061 [2]成都大学药学与生物工程学院,成都610106

出　　处：《天然产物研究与开发》2020年第2期190-199,共10页Natural Product Research and Development

基　　金：四川省科技支撑计划(2018GZ0378);国际科技交流合作提升行动计划(真菌中神经活性成分分离纯化与结构鉴定);四川省科技支撑计划(2018NFP0058)。

摘　　要：为探究海南岛部分野生灵芝的功效活性以及对野生灵芝的合理利用,分别对采集于海南岛的三株野生灵芝进行了形态和分子鉴定,并对子实体多糖的单糖组分和抗氧化活性进行了分析。结果表明,HN-1为紫芝(Ganoderma sinense)、HN-2为南方灵芝(Ganoderma australe)、HN-3为无柄紫灵芝(Ganoderma mastoporum);三株灵芝多糖的单糖组成和摩尔比存在一定的差异,HN-1多糖主要由甘露糖、木糖、鼠李糖组成,其摩尔比为1∶0.29∶0.14,HN-2多糖主要由甘露糖、葡萄糖组成,其摩尔比为1∶1.7,HN-3多糖主要由甘露糖、木糖、葡萄糖组成,其摩尔比1∶1.98∶1.8;三株灵芝的多糖都具有较强的抗氧化能力,其抗氧化能力都随着多糖质量浓度的增加而提高,HN-1的抗氧化活性最强,其次是HN-3,HN-2的抗氧化活性最弱。该研究结果为更好的保护和利用海南岛野生灵芝资源提供了基础资料,具有一定的参考价值。In order to explore the efficacy and activity of some wild Ganoderma in Hainan Island and the rational utilization of wild Ganoderma.The morphological and molecular identification of three wild Ganoderma collected from Hainan Island was carried out,and the monosaccharide composition and antioxidant capacity of fruiting polysaccharides were analyzed.The results showed that HN-1 was Ganoderma sinense,HN-2 was Ganoderma australe,HN-3 was Ganoderma mastoporum.Different Ganoderma showed a difference in the monosaccharide composition and molar ratio.HN-1 polysaccharide is mainly composed of mannose,xylose and rhamnose with a molar ratio of 1∶0.29∶0.14.The HN-2 polysaccharide is mainly composed of mannose and glucose,and the molar ratio is 1∶1.7.HN-3 polysaccharide is mainly composed of mannose,xylose and glucose,and its molar ratio is 1∶1.98∶1.8.The polysaccharides of three Ganoderma have strong antioxidant capacity,and their antioxidant capacity increases with the increase of polysaccharide concentration.HN-1 has the strongest antioxidant activity,followed by HN-3,and HN-2 has the weakest antioxidant activity.The results of this study provide basic information for better protection and utilization of wild Ganoderma resources in Hainan Island,and have certain reference value.

关 键 词：灵芝 系统进化 多糖组成 抗氧化活性 

分 类 号：Q938.2[生物学—微生物学] Q936