L-鸟氨酸发酵菌种的代谢工程研究进展  

Advances in metabolic engineering of L-ornithine producing strains

作　　者：徐心怡 张斌 吴晓玉[1] 江燕 陈雪岚 Xinyi Xu;Bin Zhang;Xiaoyu Wu;Yan Jiang;Xuelan Chen(Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources,College of Bioscience and Engineering,Jiangxi Agricultural University,Nanchang 330045,Jiangxi Province,China;Key Laboratory of Functional Small organic molecule,Ministry of Education,College of Life Sciences,Jiangxi Normal University,Nanchang 330022,Jiangxi Province,China)

机构地区：[1]江西农业大学生物科学与工程学院,江西省农业微生物资源开发利用工程实验室,江西南昌330045 [2]江西师范大学生命科学学院,功能有机小分子教育部重点实验室,江西南昌330022

出　　处：《微生物学报》2020年第3期421-430,共10页Acta Microbiologica Sinica

基　　金：江西农业大学博士科研启动基金;国家自然科学基金(31660019)。

摘　　要：L-鸟氨酸是一种非蛋白类氨基酸参与尿素代谢及生物多胺类的合成,其对人体具有治疗肝脏疾病、增强免疫力等作用,被广泛应用于医疗、保健、食品等领域。工业上生产鸟氨酸主要有化学法、酶法及工业发酵法。其中,发酵法因其生产成本及环境保护等方面的优势而逐渐成为研究的焦点。本文归纳了近年来采用基因工程技术选育鸟氨酸高产菌种最新研究进展,重点讨论了产鸟氨酸谷氨酸棒杆菌的代谢工程改造策略,并对未来的研究方向进行了预测。L-ornithine is a non-protein amino acid involved in urea metabolism and polyamines biosynthesis.Due to the positive functions in treating liver diseases and enhancing immunity,L-ornithine is widely used in medicine and food industry.Currently,L-ornithine production mainly depends on chemosynthesis,enzymatic catalysis and microbial fermentation.Among them,microbial fermentation has gradually become the focus for L-ornithine production owing to its superiority in cost and environmental protection.In this article,recent progress in the development of high L-ornithine producing strains through genetic engineering is summarized,the metabolic engineering strategies for improving L-ornithine production in Corynebacterium glutamicum are discussed,and the future direction is predicted.

关 键 词：L-鸟氨酸 代谢工程 谷氨酸棒杆菌 分子育种 

分 类 号：TQ922[轻工技术与工程—发酵工程]