Richness of sputum microbiome in acute exacerbations of eosinophilic chronic obstructive pulmonary disease  

作　　者：Yu-Jing Qi Xue-Jiao Sun Zhe Wang Yan-Fei Bin Ying-Hua Li Xiao-Ning Zhong Jing Bai Jing-Min Deng Zhi-Yi He 

机构地区：[1]Department of Pulmonary and Critical Care Medicine,The First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,China [2]Department of Pulmonary and Critical Care Medicine,The People’s Hospital of Liuzhou,Liuzhou,Guangxi 545006,China [3]Department of Pulmonary and Critical Care Medicine,The Second Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530007,China

出　　处：《Chinese Medical Journal》2020年第5期542-551,共10页中华医学杂志（英文版）

基　　金：supported by the Guangxi Key Research and Development Program of the Department of Science and Technology of Guangxi Zhuang Autonomous Region(No.GuikeAB17292044);China Soong Ching Ling Foundation Respiratory Disease Clinical Research Public Welfare Fund 2018 Chronic Obstructive Pulmonary Special Project(No.2018MZFZY-001).

摘　　要：Background:The eosinophilic chronic obstructive pulmonary disease(COPD)is known to be more sensitive to corticosteroid.The sputum microbiome has been shown to affect COPD prognosis,but its role in acute exacerbations of eosinophilic COPD is unclear.This study aimed to investigate the dynamic changes of the airway microbiome in patients with acute exacerbations of eosinophilic COPD.Methods:Fifty-seven patients with acute exacerbations of COPD from the First Affiliated Hospital of Guangxi Medical University between June 2017 and June 2018 were divided into two groups.Patients with eosinophils≥300 cells/mL in the peripheral venous blood were assigned to the eosinophilic group(Eos)and the rest to the non-eosinophilic group(Noneos).All patients received similar treatment including inhaled budesonide according to the guidelines.The induced sputum microbiome was analyzed on the 1st and 7th day of treatment using the 16S ribosomal RNA(rRNA)method.The levels of interleukin(IL)-6 and IL-8 were measured in the plasma and the sensitivity to corticosteroids was determined in isolated peripheral blood mononuclear cells.Quantitative data were compared between the two groups using the independent samples t test or Mann-Whitney U test.Categorical data were evaluated using Chi-squared test or Fisher’s exact test.Results:Twenty-six patients were classified into Eos group and 31 patients were classified into Noneos group.Prior to treatment,the alpha diversity(Shannon index)(2.65±0.63 vs.2.56±0.54,t=0.328,P=0.747)and the structure of the sputum microbiome were similar in the Eos group and the Noneos group.After 7 days of treatment,alpha diversity increased in both groups,while the microbiome richness(Ace index)was significantly lower in the Eos group(561.87±109.13 vs.767.88±148.48,t=-3.535,P=0.002).At the same time,IL-6(12.09±2.85 pg/mL vs.15.54±2.45 pg/mL,t=-4.913,P<0.001)and IL-8(63.64±21.69 pg/mL vs.78.97±17.13 pg/mL,t=-2.981,P=0.004)decreased more significantly in the Eos group,and the percentages of inhibition of IL-

关 键 词：Acute EXACERBATIONS of chronic obstructive pulmonary disease SPUTUM MICROBIOME EOSINOPHILIC CORTICOSTEROID INTERLEUKIN-8 inhibition 

分 类 号：R563[医药卫生—呼吸系统]