HS-SPME-GC-MS鉴别高良姜及大良姜  

Identification of Alpinia officinarum and Alpinia Galanga by HS-SPME-GC-MS

作　　者：李锦周 杨红兵[1] 刘毅[1] 陈成[1] 骆宇燊 LI Jin-zhou;YANG Hong-bing;LIU Yi;CHEN Cheng;LUO Yu-shen(Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学,武汉430065

出　　处：《湖北农业科学》2020年第1期135-138,共4页Hubei Agricultural Sciences

摘　　要：采用顶空固相微萃取(HS-SPME)技术萃取高良姜及大良姜中的挥发性成分,并用气相色谱-质谱(GC-MS)联用技术进行分析鉴定,再用面积归一化法计算各成分的相对含量。结果表明,两种药材共检测出128个峰,鉴定出69种成分。从高良姜中检测到61个峰,鉴定出44种成分,占挥发性成分总量的91.52%,主要成分有桉叶油醇、1,2,4a,5,6,8a-六氢-4,7-二甲基-1-(1-甲基乙基)萘、α-松油醇、塞瑟尔烯、1-石竹烯、β-蒎烯、2-茨酮等。从大良姜中检测到67个峰,鉴定出41种成分,占挥发性成分总量的78.29%,主要成分有β-倍半水芹烯、正十五烷、(E)-β-金合欢烯、1-石竹烯等。高良姜和大良姜在挥发性成分的组成和含量上有很大差异,两者不能混用。The volatile components of Alpinia officinarum and Alpinia Galanga were extracted by headspace solid phase microextraction(HS-SPME),and analyzed by gas chromatography-mass spectrometry(GC-MS),and the relative content of each component was calculated by the method of area normalization.The results showed that 128 peaks were detected,and 69 compounds were identified in two medicinal materials.In Alpinia officinarum,61 peaks were detected,and 44 components were identified,which is account for 91.52%of the total volatile components.The main substances were1,2,4a,5,6,8a-Hexahydro-4,7-dimethyl-1-(1-methylethyl)naphthalene,Eucalyptol,alpha-Terpineol,Seychellene,1-Caryophyllene,beta-Pinene,(1r,4r)-(+)-campho,etc.67 peaks were detected,and 41 components were identified from Alpinia Galanga,which is account for 78.29%of the total volatile components;the main substances wereβ-Sesquiphellandrene,Pentadecane,(E)-b-Farnesene,l-Caryophyllene.Composition and content of volatile components.The Composition and content of volatile components in Alpinia officinarum and Alpinia Galanga are very different,and they can not be mixed together.

关 键 词：高良姜 大良姜 顶空固相微萃取 气相色谱-质谱法 挥发性成分 

分 类 号：O657.72[理学—分析化学] S567.9[理学—化学]