马氏珠母贝肉酶解产物的抗酒精性肝损伤作用  

Anti-hepatic injury effect of enzymatic hydrolysate from soft tissue of Pinctada martensii

作　　者：钟佳佳 章超桦 高加龙 秦小明 曹文红 郑惠娜 林海生 ZHONG Jiajia;ZHANG Chaohua;GAO Jialong;QIN Xiaoming;CAO Wenhong;ZHENG Huina;LIN Haisheng(College of Food Science and Technology,Guangdong Ocean University/National Research and Development Branch Center for Shellfish Processing(Zhanjiang)/Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Guangdong Province Engineering Laboratory for Marine Biological Products/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China;Dalian Polytechnic University/Collaborative Innovation Center of Seafood Deep Processing,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院/国家贝类加工技术研发分中心(湛江)/广东省水产品加工与安全重点实验室/广东省海洋生物制品工程实验室/水产品深加工广东普通高等学校重点实验室,广东湛江524088 [2]大连工业大学/海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

出　　处：《南方水产科学》2020年第2期107-114,共8页South China Fisheries Science

基　　金：广东省应用型科技研发专项资金项目(2016B020235002);国家贝类产业技术体系建设专项(CARS-49);广东普通高等学校水产品高值化加工与利用创新团队项目(GDOU2016030503);海洋贝类营养健康食品关键技术及产业化(GDOU2017052606)。

摘　　要：为探究马氏珠母贝肉酶解产物(Enzymatic hydrolysate from Pinctada martensii,EP)对酒精性肝损伤(Alcoholic liver damage,ALD)的保护作用,该研究将EP超滤分级为截留分子量>10 kD(EP-Ⅰ)、3~10 kD(EP-Ⅱ)和<3 kD(EP-Ⅲ)3个组分,检测其体外抗肝损伤活性及对ALD小鼠肝保护作用的影响。体外试验结果显示,EP-Ⅲ可显著激活体外乙醇脱氢酶(ADH)活性(P<0.01),3个超滤组分均具有一定的体外抗氧化能力且EP-Ⅲ>EP-Ⅱ>EP-Ⅰ;动物试验结果显示,与模型对照组相比,各超滤组分均能够显著降低小鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)活力、小鼠肝脏指数及肝脏中丙二醛(MDA)和甘油三酯(TG)含量,同时显著增强小鼠肝脏中超氧化物歧化酶(SOD)、乙醇脱氢酶(ADH)和乙醛脱氢酶(ALDH)活力,提高肝脏中的谷胱甘肽(GSH)含量。综上,马氏珠母贝肉酶解超滤组分对急性ALD具有一定的辅助保护作用,其中EP-Ⅲ的保护作用效果最佳,其机制可能与加快机体乙醇代谢和减缓乙醇对机体造成的氧化损伤相关。In order to analyze the anti-hepatic injury effect of enzymatic hydrolysate from Pinctada martensii(EP),we seperated EP by membrane ultrafiltration into three molecular size fractions[MW>10 kD(EP-Ⅰ),MW=3–10 kD(EP-Ⅱ)and MW<3 kD(EP-Ⅲ)],and then measured the effects of three ultrafiltration fractions on the anti-ALD in vitro and liver-protection on mice.The results of experiments in vitro show that EP-III could activate alcohol dehydrogenase(ADH)significantly(P<0.01),and three fractions demonstrated different antioxidant capacities(EP-Ⅲ>EP-Ⅱ>EP-Ⅰ).The results of mice experiment show that compared with the alcohol model group,the activities of ALT and AST in serum,liver index,the levels of MDA and TG in liver decreased in each ultrafiltration fraction group significantly,while the activities of SOD,ADH and ALDH and the levels of GSH in liver increased significantly.Therefore,the ultrafiltration fractions of enzymatic hydrolysate from soft tissue of P.martensii showed good protective effect on alcoholic liver damage,and the effect of EP-Ⅲwith low molecular mass was the best.Its mechanism may be related to accelerating ethanol metabolism and slowing down the oxidative damage caused by ethanol.

关 键 词：马氏珠母贝肉酶解产物 酒精性肝损伤 体外抗肝损伤活性 乙醇代谢 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]