MicroRNA regulatory pattern in spinal cord ischemia-reperfusion injury  

作　　者：Zhi-Gang Liu Yin Li Jian-Hang Jiao Hao Long Zhuo-Yuan Xin Xiao-Yu Yang 

机构地区：[1]Department of Orthopedics,The Second Hospital of Jilin University,Changchun,Jilin Province,China [2]School of Public Health,Jilin University,Changchun,Jilin Province,China [3]Pain Clinic,The Sixth Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang Uygur Autonomous Region,China [4]The Key Laboratory of Zoonosis Search,Chinese Ministry of Education,College of Basic Medicine,Jilin University,Changchun,Jilin Province,China

出　　处：《Neural Regeneration Research》2020年第11期2123-2130,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81350013(to XYY)。

摘　　要：After spinal cord injury, dysregulated miRNAs appear and can participate in inflammatory responses, as well as the inhibition of apoptosis and axon regeneration through multiple pathways. However, the functions of miRNAs in spinal cord ischemia-reperfusion injury progression remain unclear. miRCURY LNATM Arrays were used to analyze miRNA expression profiles of rats after 90 minutes of ischemia followed by reperfusion for 24 and 48 hours. Furthermore, subsequent construction of aberrantly expressed miRNA regulatory patterns involved cell survival, proliferation, and apoptosis. Remarkably, the mitogen-activated protein kinase(MAPK) signaling pathway was the most significantly enriched pathway among 24-and 48-hour groups. Bioinformatics analysis and quantitative reverse transcription polymerase chain reaction confirmed the persistent overexpression of miR-22-3 p in both groups. These results suggest that the aberrant miRNA regulatory network is possibly regulated MAPK signaling and continuously affects the physiological and biochemical status of cells, thus participating in the regulation of spinal cord ischemia-reperfusion injury. As such, miR-22-3 p may play sustained regulatory roles in spinal cord ischemia-reperfusion injury. All experimental procedures were approved by the Animal Ethics Committee of Jilin University, China [approval No. 2020(Research) 01].

关 键 词：gene REGULATORY networks microarray analysis MICRORNA miR-22-3p MITOGEN-ACTIVATED protein kinase signaling pathway nerve REGENERATION neural REGENERATION spinal CORD ISCHEMIA-REPERFUSION injury transcriptome 

分 类 号：R651.2[医药卫生—外科学]