酵母菌-植物乳杆菌复合发酵菠萝酵素生物活性的初步研究  

Study on the bioactivity of pineapple Jiaosu fermented by yeast and Lactobacillus plantarum

作　　者：赵治巧 曾莉 万玉军[2] 王刚[2] 唐自钟[1] 布同良[1] 陈惠[1] 罗丽娟 ZHAO Zhiqiao;ZENG Li;WAN Yujun;WANG Gang;TANG Zizhong;BU Tongliang;CHEN Hui;LUO Lijuan(College of Life Science,Sichuan Agricultural University,Ya′an 625014,China;Sichuan Food and Fermentation Industry Research&Design Institute,Chengdu 611130,China)

机构地区：[1]四川农业大学生命科学学院,四川雅安625014 [2]四川省食品发酵工业研究设计院,四川成都611130

出　　处：《食品与发酵工业》2020年第7期110-115,共6页Food and Fermentation Industries

基　　金：四川省科技厅重点研发项目(2019YFG0154);四川省科技厅应用基础(重点)研发项目(2019YJ0549)。

摘　　要：为了解菠萝酵素生物活性,对菠萝酵素化学成分含量、功效酶活力和抗氧化能力进行研究。结果表明,菠萝酵素总糖、总酚、总黄酮和蛋白质含量分别为(6.67±1.5)、(0.486±0.006)、(0.083±0.007)、(0.220±0.014)mg/mL;总超氧化物歧化酶(T-SOD)、蛋白酶、脂肪酶、α-淀粉酶活力分别为(84.84±1.16)、(1.19±0.40)、(1.10±0.07)、(0.17±0.02)U/mL;以抗坏血酸(Vc)为阳性对照,在实验浓度范围内,菠萝酵素对1,1-二苯基-2-三硝基苯肼自由基、羟基自由基和超氧阴离子自由基清除能力的半抑制浓度(IC 50)分别为0.011、0.070、0.250 mg Vc当量/mL;总抗氧化能力为(31.62±1.73)U/mL。Pineapple Jiaosu is produced from pineapple fruit fermented by yeast and Lactobacillus plantarum,and is rich in nutrients.In order to evaluate the biological activity of pineapple Jiaosu,the content of chemical ingredients,enzyme activity,and antioxidant capacity were determined.The results showed that the total sugar,total phenol,total flavonoids,and protein contents were(6.67±1.5),(0.486±0.006),(0.083±0.007)and(0.220±0.014)mg/mL of pineapple Jiaosu,respectively.The activities of total superoxide dismutases(T-SOD),proteases,lipases andα-amylases were(84.84±1.16),(1.19±0.40),(1.10±0.07)and(0.17±0.02)U/mL of pineapple Jiaosu,respectively.Ascorbic acid was used as a positive control to assess the antioxidant activity of pineapple Jiaosu.Within the experimental concentration range,the half-inhibitory concentration(IC 50)of 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging activity of pineapple Jiaosu was equivalent to 0.011 mg/mL of ascorbic acid,while that of hydroxyl free radical scavenging activity 0.070 mg Vc equivalent/mL and superoxide anion free radical scavenging activity 0.250 mg Vc equivalent/mL.The total antioxidant capacity was(31.62±1.73)U/mL.The result lays a foundation for the development and utilization of pineapple Jiaosu in the future.

关 键 词：菠萝酵素 化学成分 功效酶 抗氧化能力 

分 类 号：TS255.4[轻工技术与工程—农产品加工及贮藏工程]