松针精油的协同抑菌效应及机制  

Synergistic antimicrobial effect and mechanism of pine needle essential oil

作　　者：刘文丽 包怡红[1,2] LIU Wenli;BAO Yihong(College of Forestry,Northeast Forestry University,Harbin 150040,China;Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province,Harbin 150040,China)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]黑龙江省森林食品资源利用重点实验室,黑龙江哈尔滨150040

出　　处：《南京林业大学学报（自然科学版）》2020年第2期98-104,共7页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：黑龙江省自然科学基金资助项目(ZD2019C002);国家重点研发计划(2017YFC1601901)。

摘　　要：【目的】研究3种松针精油对供试菌的协同抑菌效应和机制,利用松针精油天然抑菌物质抑制微生物的生长特性,为将松针精油应用于食品、化妆品等领域提供理论依据。【方法】通过微量二倍稀释法测定黑皮油松松针精油(PTEO)、樟子松松针精油(PSEO)、红松松针精油(PKEO)对大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)、枯草芽孢杆菌(Bacillus subtilis)的抑菌效果,通过棋盘稀释法来测定其协同效果及最佳复配比,以最佳精油复配比研究其对3种供试菌的抑菌机理。【结果】3种精油单独作用时均表现出对3种供试菌较好的抑菌效果,部分复配后效果得到明显提高,针对不同微生物复配最佳抑菌配方为:E.coli用抑菌精油为PTEO和PSEO,浓度均为0.31μL/mL;S.aureus用抑菌精油为PKEO和PTEO,浓度均为0.16μL/mL;B.subtilis用抑菌精油为PKEO和PSEO,浓度分别为0.16、0.08μL/mL。通过复配精油对3种供试菌的抑菌机理研究发现复配精油能够破坏菌体的正常形态,破坏细胞膜的渗透性,导致核酸等大分子物质的泄露,并且对细胞菌体蛋白的合成和积累有干扰作用。【结论】3种精油复配后表现出更好的抑菌作用,精油之间的协同作用可以减少到抑菌效果时精油的用量。【Objective】Synergistic bacteriostatic effects of pine needle essential oil from three pine species on bacteria and the underlying mechanism were studied;moreover,a theoretical basis was provided for the use of pine needle essential oil as a natural bacteriostatic substance to inhibit the growth of microorganisms and its application in food and cosmetics.【Method】The antibacterial effects of pine needle oil from Pinus tabulaeformis var.mukdensis(PTEO),P.sylvestris var.mongolica Litv.(PSEO),and P.koraiensis Sieb.et Zucc.(PKEO)on Escherichia coli,Staphylococcus aureus,and Bacillus subtilis were determined by using a micro-dilution method.The synergistic effects and the optimal compounding ratio were determined by using a checkerboard dilution method.The antibacterial mechanism against Escherichia Coli,Staphylococcus aureus,and Bacillus subtilis was studied using the optimal essential oil ratio.【Result】The results revealed that the three essential oils showed good antibacterial effects against the three tested bacteria.After compounding,the effect was evidently improved,and the composition of and bacteriostatic effect on different microorganisms also differed.The highest antibacterial effects after compounding were obtained with the following ratios:E.coli(PTEO and PSEO,MIC combination 0.31μL/mL each),S.aureus(PKEO and PTEO,MIC combination 0.16μL/mL each),and B.subtilis(PKEO and PSEO,MIC combination:0.16 and 0.08μL/mL,respectively).The investigation of the antibacterial mechanism of the compounded oils on the three tested bacteria indicated that compounded essential oils can disrupt the normal morphology of bacterial cells and permeability of cell membranes,leading to the leakage of macromolecules such as nucleic acids,destruction of the permeability of the cell membrane proteins;moreover,they interfere with the synthesis and accumulation of cell proteins.【Conclusion】The combination of three pine needle essential oils has a good antibacterial effect,and the synergistic effect of the essential oils c

关 键 词：松针精油 协同作用 抑菌机理 黑皮油松 樟子松 红松 

分 类 号：TS201.3[轻工技术与工程—食品科学]