团头鲂肠道菌株MA35产纤维素酶分离纯化及性质分析  

Isolation,purification and characterization of cellulase produced from Aspergillus niveus MA35 in the gut of the Megalobrama amblycephala

作　　者：江小妹 林春伟 王魁云 蒋霞云[1] 李燕[1,2,3] 邹曙明 JIANG Xiaomei;LIN Chunwei;WANG Kuiyun;JIANG Xiayun;LI Yan;ZOU Shuming(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Engineering Research Center for Processing and Storage of Shanghai Seawater Products,Shanghai 201306,China;National Freshwater Aquatic Products Processing Technology R&D Center,Shanghai 201306,China;Key Laboratory of Aquatic Genetic Resources and Utilization,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海水产品加工及贮藏工程技术研究中心,上海201306 [3]国家淡水水产品加工技术研发分中心,上海201306 [4]上海海洋大学农业农村部种质资源与利用重点开放实验室,上海201306

出　　处：《上海海洋大学学报》2020年第2期313-320,共8页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31201760,31572220);国家科技支撑计划(2015BAD17B02)。

摘　　要：对团头鲂肠道菌株Aspergillus niveus MA35发酵得到一种内切型纤维素酶采用Q-琼脂糖凝胶FF阳离子交换层析和葡聚糖G-100凝胶层析进行分离纯化。酶的比活力由22.3 U/mg提高到30.6 U/mg。SDSPAGE结果显示,酶的分子量约为45 ku。该酶水解羧甲基纤维素钠的最适温度为45℃,最适pH 4.5,在pH 4.0~8.0以及30~55℃之间具有良好的稳定性。在终离子浓度为1 mmol/L以及10 mmol/L下,Zn^2+、Mn^2+对酶的活性有激活作用,Mg^2+、Cu^2+、Fe^2+、Cd^2+、Co^2+对酶的活性有抑制作用,其中Mg^2+、Cu^2+、Fe^2+抑制作用较强,Na^+、K^+、Ca^2+对酶的活性几乎没有影响。An intracellular endo-cellulase was isolated and purified from the fermentation of Aspergillus niveus MA35 in the gut of Megalobrama amblycephala.The enzyme was purified sequentially by Q-Sepharose Fast Flow chromatography and Sephadex G-100 gel chromatography.The specific activity of the purified endoglucanase increased to 30.6 U/mg from 22.3 U/mg of the crude endoglucanase.The molecular masses of the enzyme was determined by SDS-PAGE to be about 45 ku.The optimum temperature is 45℃and the optimum pH is 4.5.The enzyme has good stability between pH 4.0-8.0 and 30-55℃.Zn^2+and Mn^2+have an activation effect on enzymes.Mg^2+,Cu^2+,Fe^2+,Cd^2+and Co^2+have inhibitory effects on enzyme activities,among which Mg^2+,Cu^2+and Fe^2+have strong inhibitory effects,and Na^+,K^+and Ca2+have little effect on enzymes.

关 键 词：纤维素酶 分离纯化 酶学性质 肠道菌株 

分 类 号：Q93[生物学—微生物学]