The Features and Regulation of Co-transcriptional Splicing in Arabidopsis  

作　　者：Danling Zhu Fei Mao Yuanchun Tian Xiaoya Lin Lianfeng Gu Hongya Gu Li-jia Qu Yufeng Wu Zhe Wu 

机构地区：[1]SUSTech-PKU Institute of Plant and Food Science,Department of Biology,Southern University of Science and Technology,Shenzhen 518055,China [2]State Key Laboratory for Crop Genetics and Germplasm Enhancement,Bioinformatics Center,Nanjing Agriculture University,Nanjing,Jiangsu 210095,China [3]State Key Laboratory of Protein and Plant Gene Research,Peking-Tsinghua Center for Life Sciences,College of Life Sciences,Peking University,Beijing 100871,China [4]Basic Forestry and Proteomics Research Center,College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China [5]Present address:School of Life Sciences,Guangzhou University,Guangzhou 510006,China

出　　处：《Molecular Plant》2020年第2期278-294,共17页分子植物（英文版）

基　　金：supported by Guangdong Innovative and Entrepreneurial Research Team Program(2016ZT06S172);the Shenzhen Sci-Tech Fund No.KYTDPT20181011104005;the National Natural Science Foundation of China(31771365 to Z.W.and 31800268 to D.Z.).

摘　　要：Precursor mRNA(pre-mRNA)splicing is essential for gene expression in most eukaryotic organisms.Previous studies from mammals,Drosophila,and yeast show that the majority of splicing events occurs co-transcriptionally.In plants,however,the features of co-transcriptional splicing(CTS)and its regulation still remain largely unknown.Here,we used chromatin-bound RNA sequencing to study CTS in Arabidopsis thaliana.We found that CTS is widespread in Arabidopsis seedlings,with a large proportion of alternative splicing events determined co-transcriptionally.CTS efficiency correlated with gene expression level,the chromatin landscape and,most surprisingly,the number of introns and exons of individual genes,but is independent of gene length.In combination with enhanced crosslinking and immunoprecipitation sequencing analysis,we further showed that the hnRNP-like proteins RZ-1B and RZ-1C promote efficient CTS globally through direct binding,frequently to exonic sequences.Notably,this general effect of RZ-1B/1C on splicing promotion is mainly observed at the chromatin level,not at the mRNA level.RZ-1C promotes CTS of multiple-exon genes in association with its binding to regions both proximal and distal to the regulated introns.We propose that RZ-1C promotes efficient CTS of genes with multiple exons through cooperative interactions with many exons,introns,and splicing factors.Our work thus reveals important features of CTS in plants and provides methodologies for the investigation of CTS and RNA-binding proteins in plants.

关 键 词：co-transcriptional SPLICING CB-RNA-seq eClip-seq RNA binding protein CHROMATIN TRANSCRIPTION 

分 类 号：Q943.2[生物学—植物学]