A Plant SMALL RNA-BINDING PROTEIN 1 Family Mediates Cell-to-Cell Trafficking of RNAi Signals  

作　　者：Yan Yan Byung-Kook Ham Yee Hang Chong Shyi-Dong Yeh William J.Lucas 

机构地区：[1]Department of Plant Biology,College of Biological Sciences,University of California,Davis,CA 95616,USA [2]Department of Plant Pathology,National Chung-Hsing University,Taichung [3]Present address:Global Institute for Food Security,University of Saskatchewan,Saskatoon,SK S7N 4J8,Canada [4]Present address:Department of Biology,University of Saskatchewan,112 Science Place,Saskatoon,SK S7N 5E2,Canada

出　　处：《Molecular Plant》2020年第2期321-335,共15页分子植物（英文版）

基　　金：provided by the National Science Foundation(IOS-1339128 to W.J.L.).Y.H.C.was supported,in part,by a graduate fellowship provided by the National Chung-Hsing University,Taichung,China.

摘　　要：In plants,RNA interference(RNAi)plays a pivotal role in growth and development,and responses to environmental inputs,including pathogen attack.The intercellular and systemic trafficking of small interfering RNA(siRNA)/microRNA(miRNA)is a central component in this regulatory pathway.Currently,little is known with regards to the molecular agents involved in the movement of these si/miRNAs.To address this situation,we employed a biochemical approach to identify and characterize a conserved SMALL RNA-BINDING PROTEIN 1(SRBP1)family that mediates non-cell-autonomous small RNA(sRNA)trafficking.In Arabidopsis,AtSRBP1 is a glycine-rich(GR)RNA-binding protein,also known as AtGRP7,which we show binds single-stranded siRNA.A viral vector,Zucchini yellow mosaic virus(ZYMV),was employed to functionally characterized the AtSRBP1-4(AtGRP7/2/4/8)RNA recognition motif and GR domains.Cellular-based studies revealed the GR domain as being necessary and sufficient for SRBP1 cell-to-cell movement.Taken together,our findings provide a foundation for future research into the mechanism and function of mobile sRNA signaling agents in plants.

关 键 词：RNAI SMALL RNA-BINDING PROTEIN FAMILY SMALL RNA movement non-cell-autonomous PROTEIN glycinerich PROTEIN 

分 类 号：Q943.2[生物学—植物学]