保健食品中低聚木糖高效液相色谱-示差折光法测定  

Determination of xylo-oligosaccharides in health foods by high performance liquid chromatography with refractive index

作　　者：郑凤家[1] 李蔚[1] 陈金东[1] 姜大峰[1] 李凤华[1] 王晓琳 ZHENG Feng-jia;LI Wei;CHEN Jin-dong(Shandong Provincial Center for Disease Control and Prevention,Ji'nan,Shandong Province 250014,China)

机构地区：[1]山东省疾病预防控制中心

出　　处：《中国公共卫生》2020年第3期429-432,共4页Chinese Journal of Public Health

摘　　要：目的建立高效液相色谱硫酸水解法测定保健食品中低聚木糖(以木糖计)的方法。方法样品经水提取30 min,低温下利用乙醇沉降大分子糖类,硫酸水解90 min,用氢氧化钠中和后,氨基柱分离,示差折光检测器检测,外标法定量,水解前后木糖含量之差即低聚木糖含量。结果木糖浓度为0.25~5.00 mg/mL时,其质量浓度与峰面积线性关系良好(Y=61349.49X-1028.52,r=0.9999)。在保健食品中加标回收率为83.5%~96.7%,相对标准偏差(RSD)为1.67%~4.82%。低聚木糖检出限为1.1 g/kg,定量限为3.3 g/kg。结论该方法准确、灵敏、方便、能够测定低聚木糖终端产品中的含量。Objective To develop a method for the determination of xylo-oligosaccharides(in xylose)in health foods by high performance liquid chromatography with refractive index(HPLC-RI).Methods The samples were extracted with water for 30 minutes then higher molecular saccharides were precipitated with alcohol at low temperature.After subsequently hydrolyzed with sulfuric acid solution for 90 minutes,neutralized with sodium hydroxide solution and separated on a NH2 column,the samples were detected quantitatively with refractive index detector using external standard.The xylooligosaccharides content was measured based on the difference value for xylose concentration before and after the hydrolysis process.Results A high linearity of the calibration curve between the peak area and the mass concentration of xylose was observed(Y=61349.49 X-1028.52,r=0.9999)under the xylose concentration ranging between 0.25-5.00 mg/mL.The recovery rates were between 83.5% and 96.7% and the relative standard deviations ranged from 1.67%to 4.82%in health foods.The detection limit of the method is 1.1 g/kg and the quantification limit is 3.3 g/kg.Conclusion The established method is accurate,sensitive,and simple and could be used for the determination of xylo-oligosaccharides in health foods.

关 键 词：高效液相色谱 低聚木糖 保健食品 

分 类 号：R115[医药卫生—公共卫生与预防医学]