Dehydrocostus lactone exerts the antitumor effect in non-small cell lung cancer H1299 cells  

作　　者：Chao-Yue Su Hui Wang Yan-Rui Pan Ling-Ling Zhang Qiao-Ru Guo Yan-Yan Yan Jia-Jun Li Jia-Li Fu Xin-YueFan Yu-Qing Wang Jian-Ye Zhang 

机构地区：[1]Guangdong Provincial Key Laboratory of Molecular Target&Clinical Pharmacology,School of Pharmaceutical Sciences and the Fifth Affiliated Hospital,Guangzhou Medical University,Guangzhou 511436 [2]China.2Guangzhou Institute of Pediatrics/Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou 510623 [3]China.3Institute of Respiratory and Occupational Diseases,Collaborative Innovation Center for Cancer,Medical College,Shanxi Datong University,Datong 037009 [4]China.4Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Medical University,Haikou,571199,P.R.China

出　　处：《TMR Cancer》2020年第3期101-111,共11页TMR肿瘤

基　　金：supported by the National Natural Science Foundation of China(U1903126 and 81903467);Fund of Guangzhou Science and Technology Program(201707010048);Guangdong Basic and Applied Basic Research Foundation(2020A1515010005 and 2020A1515010605);Fund of Undergraduate Innovation Project of Guangzhou Medical University(2019A086).

摘　　要：Background:Dehydrocostus lactone(DHC),a sesquiterpene lactone derived from Aucklandiae Radix,has been proved to possess various pharmacological activities.Recently,studies have reported that DHC has potential antitumor activity.However,few studies have reported the effect of DHC on non-small cell lung cancer(NSCLC).Here,we investigated the antitumor effect of DHC in NSCLC H1299 cells.Methods:MTT assay and colony formation assay was used to assess the anti-proliferation effects of DHC in H1299 cells.Wound healing and Transwell assays were utilized to determine the inhibitory effects of migration and invasion,respectively.Apoptosis was detected using the Annexin V/propidium iodide test.Real-time-quantitative PCR(RT-qPCR)was used to detect the mRNA expression level.Results:We demonstrated here that DHC inhibited the proliferation,migration and invasion of H1299 cells in a dose-or time-dependent manner.Additionally,after treating with DHC at the concentration of 32.0μM,the apoptosis of H1299 cells was significantly induced.Moreover,DHC affected the mRNA expression of E-cadherin,N-cadherin,Snail,c-Myc,integrinα2,Survivin and matrix metalloproteinase 2.Conclusion:To summarize,our data support that DHC can inhibit proliferation,invasion,migration and induced apoptosis of NSCLC H1299 cells.DHC should be considered for its potential for adjuvant therapeutic development.

关 键 词：Dehydrocostus LACTONE NON-SMALL cell LUNG cancer Apoptosis MIGRATION INVASION 

分 类 号：R73[医药卫生—肿瘤]