超高效液相色谱-串联质谱法测定猪肉中93种兽药和违禁化合物  

Determination of 93 veterinary drugs and prohibited chemicals in pork by ultra high performance liquid chromatography-tandem mass spectrometry

作　　者：姜文华 韦立毅 邱晋 尹伟丽 杨懿 孙成均 JIANG Wen-hua;WEI Li-yi;QIU Jin;YIN Wei-li;YANG Yi;SUN Cheng-jun(West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu,Sichuan 610041,China;不详)

机构地区：[1]四川大学华西公共卫生学院/华西第四医院,四川成都610041 [2]四川仲测质量技术检测有限公司,四川成都610015 [3]西南医科大学公共卫生学院,四川泸州646000

出　　处：《现代预防医学》2020年第8期1477-1483,共7页Modern Preventive Medicine

摘　　要：目的建立猪肉中93种兽药和违禁化合物残留的超高效液相色谱-串联质谱测定方法。方法样品用0.005mol/L EDTA-乙腈-甲醇(3∶1, v/v,含1%乙酸)混合溶液提取后,冷冻除酯,再经壳聚糖净化,经稀释后,用超高效液相色谱-串联质谱法测定。结果方法线性范围为0.05~200.00μg/L,定量限为0.266~50.100μg/kg,回收率为64.6%~118.6%,日内和日间RSD分别为0.50%~15.90%和0.18%~19.20%。结论本法简便快速、灵敏、准确,可用于猪肉中93种兽药和违禁成分的测定。Objective To establish a method for the determination of 93 veterinary drugs and prohibited chemicals in pork by ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS). Methods After the samples were extracted with 0.005 mol/L EDTA and acetonitrile-methanol(3:1, v/v, containing 1% acetic acid), then frozen for removing the fat and then purified with chitosan. The purified solution was diluted and the target analytes were determined by UHPLC-MS/MS. Results The linear ranges of the method were 0.05-200 μg/L. The limit of quantification was 0.266-50.1 μg/kg, and the recoveries were 64.6%-118.6%. The RSDs of intra-day and inter-day were 0.50%-15.9% and 0.18%-19.2%, respectively.Conclusion The method is simple, quick, sensitive and accurate. It can be used for the determination of 93 veterinary drugs and prohibited chemicals in pork.

关 键 词：超高效液相色谱-串联质谱法 兽药残留 违禁成分 猪肉 

分 类 号：R115[医药卫生—公共卫生与预防医学]